瞬时受体电位M型（TRPM）基因在心肌缺血再灌注中的表达评估。
Evaluation of TRPM (transient receptor potential melastatin) genes expressions in myocardial ischemia and reperfusion.
作者信息
Demir Tuncer, Yumrutas Onder, Cengiz Beyhan, Demiryurek Seniz, Unverdi Hatice, Kaplan Davut Sinan, Bayraktar Recep, Ozkul Nadide, Bagcı Cahit
机构信息
Department of Medical Physiology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
出版信息
Mol Biol Rep. 2014 May;41(5):2845-9. doi: 10.1007/s11033-014-3139-0. Epub 2014 Jan 21.
In the present study, the expression levels of TRPM1, TRPM2, TRPM3, TRPM4, TRPM5, TRPM6, TRPM7, and TRPM8 genes were evaluated in heart tissues after ischemia/reperfusion (IR). For this study, 30 albino male Wistar rats were equally divided into three groups as follows: Group 1: control group (n:10), Group II: ischemia group (ischemia for 60 min) (n:10) and Group III: IR (reperfusion 48 h after ischemia for 60 min and reperfusion for 48 h). The expression levels of the TRPM genes were analyzed by semi-quantitative reverse transcriptase-PCR. When compared to the ischemia control, the expression levels of TRPM2, TRPM4, and TRPM6 did not change, whereas that of TRPM7 increased. However, TRPM1, TRPM3, TRPM5, and TRPM8 were not expressed in heart tissue. Histopathological analysis of the myocardial tissues showed that the structures that were most damaged were those exposed to IR. The findings showed that there is a positive relationship between TRPM7 expression and myocardial IR injury.
在本研究中，评估了缺血/再灌注（IR）后心脏组织中瞬时受体电位阳离子通道M亚家族成员1（TRPM1）、瞬时受体电位阳离子通道M亚家族成员2（TRPM2）、瞬时受体电位阳离子通道M亚家族成员3（TRPM3）、瞬时受体电位阳离子通道M亚家族成员4（TRPM4）、瞬时受体电位阳离子通道M亚家族成员5（TRPM5）、瞬时受体电位阳离子通道M亚家族成员6（TRPM6）、瞬时受体电位阳离子通道M亚家族成员7（TRPM7）和瞬时受体电位阳离子通道M亚家族成员8（TRPM8）基因的表达水平。在本研究中，30只白化雄性Wistar大鼠被平均分为三组，如下：第1组：对照组（n = 10），第II组：缺血组（缺血60分钟）（n = 10），第III组：IR组（缺血60分钟后再灌注48小时，再灌注48小时）。通过半定量逆转录聚合酶链反应分析TRPM基因的表达水平。与缺血对照组相比，TRPM2、TRPM4和TRPM6的表达水平没有变化，而TRPM7的表达水平增加。然而，TRPM1、TRPM3、TRPM5和TRPM8在心脏组织中未表达。心肌组织的组织病理学分析表明，受损最严重的结构是暴露于IR的结构。研究结果表明，TRPM7表达与心肌IR损伤之间存在正相关关系。
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