美国猪博卡病毒的检测与特性分析
Detection and characterization of porcine bocavirus in the United States.
作者信息
Huang Jinhai, Mor Sunil K, Erber Jonathan, Voss Elyce, Goyal Sagar M
机构信息
Department of Veterinary Population Medicine and Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Ave, St. Paul, MN, 55108, USA.
出版信息
Arch Virol. 2014 Jul;159(7):1797-801. doi: 10.1007/s00705-013-1972-4. Epub 2014 Jan 21.
We screened pigs (n = 203) presenting with respiratory illness or diarrhea for porcine bocavirus (PBoV); 88 (43.30 %) were positive by PCR. More positives were seen in diarrhea cases (48.7 %) than in respiratory cases (29.1 %). Based on phylogenetic analysis of 540 nucleotides of the NS1 gene, the viruses could be divided into four possible groups. Group IV sequences did not match any GenBank sequences, while groups I, II and III gave matches with PBoV3, PBoV4 and PBoV5, respectively. The wide range (70 % to 100 %) of nucleotide (nt) sequence identity among strains in this study indicates high genetic diversity among porcine bocaviruses.
我们对出现呼吸道疾病或腹泻症状的猪（n = 203）进行了猪博卡病毒（PBoV）筛查；88头（43.30%）通过聚合酶链反应（PCR）检测呈阳性。腹泻病例中的阳性率（48.7%）高于呼吸道病例（29.1%）。基于对NS1基因540个核苷酸的系统发育分析，这些病毒可分为四个可能的组。第四组序列与任何基因库序列均不匹配，而第一组、第二组和第三组分别与PBoV3、PBoV4和PBoV5匹配。本研究中各毒株之间核苷酸（nt）序列同一性范围较广（70%至100%），表明猪博卡病毒之间存在高度的遗传多样性。
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