Keros Tomislav, Jemeršić Lorena, Toplak Ivan, Prpić Jelena
1 Department of Virology, Croatian Veterinary Institute , Savska cesta 143, HR-10 000 Zagreb , Croatia.
2 Institute of Microbiology and Parasitology, Virology Unit, Veterinary Faculty, University of Ljubljana , Ljubljana , Slovenia.
Acta Vet Hung. 2017 Dec;65(4):565-573. doi: 10.1556/004.2017.055.
A survey was conducted to evaluate the presence and prevalence of Porcine Bocavirus (PBoV) in Croatian domestic pigs by means of PCR targeting the NS1 gene fragment of PBoV. This study included testing of faecal samples collected from 10 small commercial farms and 11 small backyard holdings in Croatia. The presence of PBoV was confirmed by PCR in 24 out of 57 composite faecal samples from small commercial farms and in 12 out of 43 composite faecal samples from small backyard holdings. The PCR products of 18 positive samples were sequenced for genotyping. PBoV sequences grouped into the PBoV-a, PBoV-b and PBoV-c groups with 90.81% to 99.25% nucleotide identity. All Croatian PBoV sequences showed a high nucleotide and amino acid identity with PBoV sequences from China and Hong Kong, the United States, Sweden, and Slovenia. These results clearly show that PBoV is circulating among the domestic pig population in Croatia.
通过针对猪博卡病毒（PBoV）NS1基因片段的聚合酶链反应（PCR），对克罗地亚家猪中PBoV的存在情况和流行率进行了一项调查。本研究包括对从克罗地亚10个小型商业农场和11个小型后院养殖场采集的粪便样本进行检测。在来自小型商业农场的57份混合粪便样本中，有24份通过PCR确认存在PBoV；在来自小型后院养殖场的43份混合粪便样本中，有12份确认存在PBoV。对18份阳性样本的PCR产物进行测序以进行基因分型。PBoV序列分为PBoV-a、PBoV-b和PBoV-c组，核苷酸同一性为90.81%至99.25%。所有克罗地亚PBoV序列与来自中国、中国香港、美国、瑞典和斯洛文尼亚的PBoV序列显示出高度的核苷酸和氨基酸同一性。这些结果清楚地表明，PBoV在克罗地亚的家猪群体中传播。
