Mohan Jacob Daniel, Lee Chee Yien, Arshad Siti Suri, Selvarajah Gayathri Thevi, Bande Faruku, Ong Bee Lee, Ooi Peck Toung
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400, Serdang, Selangor, Malaysia.
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400, Serdang, Selangor, Malaysia.
Trop Anim Health Prod. 2018 Apr;50(4):733-739. doi: 10.1007/s11250-017-1489-z. Epub 2017 Dec 14.
Several strains of porcine bocaviruses have been reported worldwide since their first detection in Sweden in 2009. Subsequently, the virus has been reported to be associated with gastrointestinal and respiratory signs in weaner and grower pigs. Although Malaysia is host to a self-sufficient swine livestock industry, there is no study that describes porcine bocavirus in the country. This report is the first to describe porcine bocavirus (PBoV) in Malaysian swine herds. PBoV was identified in various tissues from sick and runt pigs using the conventional PCR method with primers targeting conserved regions encoding for the nonstructural protein (NS1) gene. Out of 103 samples tested from 17 pigs, 32 samples from 15 pigs were positive for porcine bocavirus. In addition, a higher detection rate was identified from mesenteric lymph nodes (52.9%), followed by tonsil (37.0%), and lungs (33.3%). Pairwise comparison and phylogenetic analyses based on a 658-bp fragment of NS1 gene revealed that the Malaysian PBoV strains are highly similar to PBoV3 isolated in Minnesota, USA. The presence of porcine bocavirus in Malaysia and their phylogenetic bond was marked for the first time by this study. Further studies will establish the molecular epidemiology of PBoV in Malaysia and clarify pathogenicity of the local isolates.
自2009年在瑞典首次发现以来，全球已报告了几种猪博卡病毒毒株。随后，有报道称该病毒与断奶仔猪和生长猪的胃肠道及呼吸道症状有关。尽管马来西亚拥有自给自足的养猪业，但该国尚无描述猪博卡病毒的研究。本报告首次描述了马来西亚猪群中的猪博卡病毒（PBoV）。使用针对编码非结构蛋白（NS1）基因保守区域的引物，通过常规PCR方法在患病猪和发育不良猪的各种组织中鉴定出PBoV。在从17头猪采集的103份样本中，有15头猪的32份样本检测出猪博卡病毒呈阳性。此外，肠系膜淋巴结的检出率较高（52.9%），其次是扁桃体（37.0%）和肺（33.3%）。基于NS1基因658 bp片段的成对比较和系统发育分析表明，马来西亚的PBoV毒株与在美国明尼苏达州分离出的PBoV3高度相似。本研究首次标记了马来西亚猪博卡病毒的存在及其系统发育关系。进一步的研究将确定马来西亚PBoV的分子流行病学，并阐明当地分离株的致病性。
