The Excellence Center for Animal Sciences and Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, 91775-1163, Iran,
Biol Trace Elem Res. 2014 Mar;157(3):224-33. doi: 10.1007/s12011-013-9879-1. Epub 2014 Jan 22.
Three hundred female broilers were assigned to five groups with six replicates and were fed with either a basal diet (two control groups) or the basal diet supplemented with 800-mg vitamin C/kg (Vit C group), 1,200-μg Cr(+3) from chromium (Cr) chloride/kg (Cr group) or 800-mg Vit C and 1,200-μg Cr(+3) from Cr chloride/kg (Vit C + Cr group) from 42 to 49 days of age. Treatments did not affect performance. Transport decreased insulin level in the control and Cr groups and increased glucose/insulin (G/I) ratio in the groups. The level of insulin was higher in the Vit C + Cr group than those in the control and Cr groups after the transport. The G/I ratio was lowest in the Vit C + Cr group after the transport. The transport significantly decreased triiodothyronine (T3) concentration in the groups except the Vit C + Cr group and only increased thyroxin (T4) concentration in the Vit C + Cr group. The T3/T4 ratio was significantly decreased in the groups except the Cr group by transport. The T3/T4 ratio was greatest in the Vit C + Cr group before the transport. Alkaline phosphatase activity was decreased in the Vit C + Cr group due to transport. Transport decreased triglyceride levels in the groups and also decreased low-density lipoprotein cholesterol levels in the Vit-C-supplemented groups. Transport increased malondialdehyde concentration in the control and Vit C groups and also increased glutathione peroxidase (GPx) activity in the Cr-fed groups. The GPx activity was higher in the Vit C + Cr group than those in the control and Cr groups after the transport. Ferric reducing/antioxidant power (FRAP) value was decreased in the Vit C and Cr groups by transport. Either alone or in combination, Cr increased the FRAP value before the transport. Neither transport nor treatments had significant effects on the duration of tonic immobility (TI) and number of inductions to induce TI.
300 只母鸡被分配到 5 个组，每组 6 个重复，分别用基础日粮（两组对照）或基础日粮加 800mg/kg 维生素 C（Vit C 组）、1200μg 三价铬（Cr）来自氯化铬（Cr 组）或 800mgVitC 和 1200μg 三价铬（Cr）来自氯化铬（VitC+Cr 组）从 42 到 49 天。处理不影响性能。运输降低了对照组和 Cr 组的胰岛素水平，并增加了葡萄糖/胰岛素（G/I）比值在组。维生素 C+Cr 组在运输后胰岛素水平高于对照组和 Cr 组。运输后，VitC+Cr 组的 G/I 比值最低。运输显著降低了各组的三碘甲状腺原氨酸（T3）浓度，除了 VitC+Cr 组，只有 VitC+Cr 组增加了甲状腺素（T4）浓度。运输使各组除 Cr 组外的 T3/T4 比值显著降低。在运输前，VitC+Cr 组的 T3/T4 比值最大。由于运输，碱性磷酸酶活性在 VitC+Cr 组中降低。运输降低了各组的甘油三酯水平，也降低了 VitC 补充组的低密度脂蛋白胆固醇水平。运输增加了对照组和 VitC 组的丙二醛浓度，也增加了 Cr 喂养组的谷胱甘肽过氧化物酶（GPx）活性。在运输后，VitC+Cr 组的 GPx 活性高于对照组和 Cr 组。运输降低了 VitC 和 Cr 组的铁还原/抗氧化能力（FRAP）值。在运输前，单独或联合使用 Cr 增加了 FRAP 值。无论是运输还是处理都对强直不动的持续时间（TI）和诱导 TI 的诱导次数都没有显著影响。
