Du F, Charnay Y, Dubois P
CNRS UA 559, Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, Oullins, France.
J Comp Neurol. 1987 Sep 15;263(3):436-54. doi: 10.1002/cne.902630309.
The development and distribution of substance P (SP) immunoreactivity were studied in the spinal cord and ganglia of embryonic and newly hatched chick by using the indirect immunofluorescence method. Substance P immunoreactivity was first detected in the spinal cord at embryonic stages 18-20 (incubation day 3). Before stage 32 (day 7), it was mainly found in regions corresponding to the dorsolateral funiculus and Lissauer's tract. Subsequently, SP fibers appeared in the dorsal horn. By stage 38 (day 11), they were demonstrated almost throughout the gray matter, but mostly in laminae I and II. During this period, however, many SP-positive cells were found just ventral to the central canal at the thoracic level, although a few were also detected in other areas throughout the cord. In the white matter, very dense longitudinal SP fibers were observed in Lissauer's tract and the dorsolateral funiculus, where extremely dense plexuses of SP immunoreactivity were also detected around a group of nonimmunoreactive cell bodies. At later stages, no remarkable differences were noticed in the distribution of SP fibers, but the SP-positive cells decreased gradually in number and disappeared after hatching. However, they reappeared following colchicine treatment. In the spinal ganglia, SP immunoreactivity appeared initially at stage 25 (day 4). It was mostly located in small neurons of the mediodorsal region. These cells also decreased in number from later stages but increased by colchicine treatment after hatching. The development and distribution of SP immunoreactivity in the spinal cord and ganglia were generally comparable at all levels examined, except where indicated.
采用间接免疫荧光法研究了胚胎期和新孵出雏鸡脊髓和神经节中P物质（SP）免疫反应性的发育和分布情况。在胚胎期18 - 20（孵化第3天）时，首次在脊髓中检测到P物质免疫反应性。在第32阶段（第7天）之前，它主要存在于对应于背外侧索和Lissauer束的区域。随后，SP纤维出现在背角。到第38阶段（第11天），它们几乎遍布整个灰质，但主要在I层和II层。然而，在此期间，在胸段脊髓中央管腹侧发现了许多SP阳性细胞，尽管在脊髓其他区域也检测到了少数阳性细胞。在白质中，在Lissauer束和背外侧索中观察到非常密集的纵向SP纤维，在一群非免疫反应性细胞体周围也检测到了极其密集的SP免疫反应性丛。在后期，SP纤维的分布没有明显差异，但SP阳性细胞数量逐渐减少并在孵化后消失。然而，秋水仙碱处理后它们又重新出现。在脊髓神经节中，SP免疫反应性最初在第25阶段（第4天）出现。它主要位于中背侧区域的小神经元中。这些细胞数量从后期也开始减少，但孵化后经秋水仙碱处理数量增加。在所检查的所有水平上，脊髓和神经节中SP免疫反应性的发育和分布总体上是可比的，除另有说明外。
