Du F, Dubois P M
CNRS UA 559, Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, Oullins, France.
Neuroscience. 1988 Oct;27(1):251-66. doi: 10.1016/0306-4522(88)90235-7.
The development and distribution of methionine-enkephalin-immunoreactive elements were studied in the chicken spinal cord with the indirect immunofluorescence method. Methionine-enkephalin-like immunoreactivity was first detected in the chick spinal cord at embryonic stages 29-30 (incubation day 6). Before stage 35 (day 9), it was mainly observed in fibres almost throughout the white matter. Subsequently, fibres containing the peptide appeared in the ventral half of the gray matter, but mostly in the lateral portion of the neck of the dorsal horn. From stage 40 (day 13 or 14), fibres were especially noticed in laminae 1 and 2, and in the area dorsal to the central canal. In particular, many enkephalin-immunoreactive perikarya were observed in several spinal areas during this period. Such a distribution of both enkephalin-immunoreactive fibres and perikarya remained visible at later embryonic stages, but labelled cells gradually decreased in number and disappeared after hatching. With colchicine treatment, however, a similar distribution of the peptide was found in the spinal cord of adult chickens. As in the embryo, enkephalin-immunoreactive perikarya were mainly observed in the lateral portion of the neck of the dorsal horn, in lamina 1, and in the nucleus of the dorsolateral funiculus throughout the spinal cord. At the thoracic level, many were also located ventral to the central canal. Enkephalin-immunoreactive fibres increased notably in the gray matter of adult chickens. They mainly occurred in laminae 1 and 2, in the lateral portion of the neck of the dorsal horn, and in the area around, especially dorsal to, the central canal. In contrast, enkephalin-immunoreactive fibres decreased in the white matter and they were mainly observed in the dorsolateral funiculus, in Lissauer's tract, and in the lateral funiculus adjacent to the gray. The distribution of enkephalin-immunoreactive fibres was generally comparable at all spinal levels examined. In addition, examination of post-hatched chickens showed virtually the same results as in the adult.
采用间接免疫荧光法研究了甲硫氨酸脑啡肽免疫反应性成分在鸡脊髓中的发育和分布情况。在胚胎发育第29 - 30阶段（孵化第6天）首次在鸡脊髓中检测到甲硫氨酸脑啡肽样免疫反应性。在第35阶段（第9天）之前，主要在几乎整个白质的纤维中观察到它。随后，含肽纤维出现在灰质的腹侧半部，但大多在背角颈部的外侧部分。从第40阶段（第13或14天）开始，在板层1和2以及中央管背侧区域特别注意到纤维。在此期间，在几个脊髓区域观察到许多脑啡肽免疫反应性核周体。脑啡肽免疫反应性纤维和核周体的这种分布在胚胎后期仍然可见，但标记细胞数量逐渐减少并在孵化后消失。然而，用秋水仙碱处理后，在成年鸡脊髓中发现了该肽的类似分布。与胚胎一样，脑啡肽免疫反应性核周体主要在背角颈部的外侧部分、板层1以及整个脊髓背外侧索核中观察到。在胸段水平，许多也位于中央管腹侧。成年鸡灰质中的脑啡肽免疫反应性纤维显著增加。它们主要出现在板层1和2、背角颈部的外侧部分以及中央管周围，尤其是背侧区域。相比之下，白质中的脑啡肽免疫反应性纤维减少，主要在背外侧索、利绍尔束以及与灰质相邻的外侧索中观察到。在所检查的所有脊髓水平，脑啡肽免疫反应性纤维的分布总体上是可比的。此外，对孵化后鸡的检查结果与成年鸡几乎相同。
