Tu F, Pan Z X, Yao Y, Liu H L, Liu S R, Xie Z, Li Q F
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Animal Husbandry and Veterinary Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, China.
Genet Mol Res. 2014 Jan 14;13(2):2504-12. doi: 10.4238/2014.January.14.6.
During ovarian follicular growth and development, only a few follicles actually ovulate. Recently, it was found that follicular atresia is triggered by granulosa cell apoptosis, but the molecular mechanism of follicular atresia was not understood. Using flow cytometry, we found that miR-34a promotes granulosa cell apoptosis in pig ovarian follicles. In addition, inhibin beta B was found to be a miR-34a target gene, based on luciferase reporter assays, quantitative RT-PCR and Western blotting. Taken together, our data indicate that miR-34a plays an important role in granulosa cell apoptosis by targeting the INHBB gene in the porcine ovary.
在卵巢卵泡生长和发育过程中，实际上只有少数卵泡会排卵。最近，人们发现卵泡闭锁是由颗粒细胞凋亡引发的，但卵泡闭锁的分子机制尚不清楚。通过流式细胞术，我们发现miR-34a可促进猪卵巢卵泡中的颗粒细胞凋亡。此外，基于荧光素酶报告基因检测、定量RT-PCR和蛋白质免疫印迹分析，发现抑制素βB是miR-34a的靶基因。综上所述，我们的数据表明，miR-34a通过靶向猪卵巢中的INHBB基因，在颗粒细胞凋亡中发挥重要作用。
