miR-92a 通过靶向 Smad7 基因抑制猪卵巢颗粒细胞凋亡。
MiR-92a inhibits porcine ovarian granulosa cell apoptosis by targeting Smad7 gene.
机构信息
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China.
出版信息
FEBS Lett. 2014 Nov 28;588(23):4497-503. doi: 10.1016/j.febslet.2014.10.021.
Smad7 has a key role in apoptosis of mammalian ovarian granulosa cells (GCs), as it antagonizes and fine-tunes transforming growth factor β (TGFβ) signaling. This study demonstrates that miR-92a regulates GC apoptosis in pig ovaries by targeting Smad7 directly. The expression level of miR-92a was down-regulated in atretic porcine follicles, whereas miR-92a expression led to inhibition of GC apoptosis. The Smad7 gene was identified as a direct target of miR-92a using a dual-luciferase reporter assay. Transfection of GCs with miR-92a mimics decreased Smad7 mRNA and protein levels, whereas expression of an miR-92a inhibitor in GCs had the opposite effect. In addition, knockdown of Smad7 prevented GC apoptosis in cells that expressed the miR-92a inhibitor.
Smad7 在哺乳动物卵巢颗粒细胞 (GC) 的凋亡中起关键作用，因为它拮抗并微调转化生长因子 β (TGFβ) 信号。本研究表明，miR-92a 通过直接靶向 Smad7 来调节猪卵巢中的 GC 凋亡。miR-92a 在闭锁的猪卵泡中的表达水平下调，而 miR-92a 的表达导致 GC 凋亡受到抑制。通过双荧光素酶报告基因检测鉴定出 Smad7 基因为 miR-92a 的直接靶标。用 miR-92a 模拟物转染 GC 可降低 Smad7 mRNA 和蛋白水平，而在 GC 中表达 miR-92a 抑制剂则产生相反的效果。此外，在表达 miR-92a 抑制剂的细胞中敲低 Smad7 可防止 GC 凋亡。
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