Curr Opin Plant Biol. 2013 Dec;16(6):710-7. doi: 10.1016/j.pbi.2013.10.004.
Myosins are motor proteins that drive movements along actin filaments and have long been assumed to be responsible for cytoplasmic streaming in plant cells. This conjecture is now firmly established by genetic analysis in the reference species, Arabidopsis thaliana. This work and similar approaches in the moss, Physcomitrella patens, also established that myosin-driven movements are necessary for cell growth and polarity, organelle distribution and shape, and actin organization and dynamics. Identification of a mechanistic link between intracellular movements and cell expansion has proven more challenging, not the least because of the high level of apparent genetic redundancy among myosin family members. Recent progress in the creation of functional complementation constructs and identification of interaction partners promises a way out of this dilemma.
肌球蛋白是一种沿肌动蛋白丝运动的马达蛋白,长期以来一直被认为是植物细胞质流的原因。这一假说现在已通过模式物种拟南芥的遗传分析得到了确凿证实。这项工作以及在苔藓植物中相似的方法也证明了肌球蛋白驱动的运动对于细胞生长和极性、细胞器分布和形状以及肌动蛋白的组织和动力学都是必要的。在细胞内运动和细胞扩张之间建立机制联系的证明更具挑战性,其中一个原因就是肌球蛋白家族成员之间明显存在高水平的遗传冗余。最近在构建功能互补构建体和鉴定相互作用伙伴方面的进展有望解决这一困境。