Kelley Jessica, Sharkey Leslie C, Christopherson Pete W, Rendahl Aaron
Veterinary Clinical Sciences Department, University of Minnesota, St. Paul, MN, USA.
Vet Clin Pathol. 2014 Mar;43(1):43-9. doi: 10.1111/vcp.12116. Epub 2014 Jan 21.
Models for the regulation of thrombopoiesis predict that platelet mass is the biologically regulated variable, yet clinical evaluations of thrombopoiesis are often based on platelet number. When thrombocytopenia and variation in platelet size occur concurrently, platelet count may poorly estimate total platelet mass, confounding clinical decision making.
We hypothesized that plateletcrit (PCT) provides clinically important information when platelet number is an incomplete representation of platelet mass, such as in genetic macrothrombocytopenia.
We retrospectively compared platelet count and PCTs with general reference intervals for 4 groups of dogs: sick Cavalier King Charles Spaniels (CKCS) and Greyhounds, presented for nonhematopoietic disease to the University of Minnesota (measured using an Advia 2120) and Auburn University Teaching Hospitals (measured using an Advia 120) over a 3-year period.
A canine PCT reference interval of 0.129-0.403% was established. None of the 4 sample groups had significantly more individuals below the reference interval for plateletcrit. For platelet count, only the 2 CKCS groups had significantly more individuals below the reference interval than predicted.
Use of the PCT as determined by the Advia 120/2120 appeared to avoid overestimation of low platelet mass in sick CKCS in a clinical setting. In contrast, the PCT performed similarly to the platelet count in evaluation of platelet mass in sick Greyhounds. Evaluation of the PCT should be considered in other conditions associated with increased mean platelet volume.
血小板生成调节模型预测血小板总量是生物学调节变量，但血小板生成的临床评估通常基于血小板数量。当血小板减少和血小板大小变异同时出现时，血小板计数可能无法准确估计血小板总量，从而混淆临床决策。
我们假设当血小板数量不能完全代表血小板总量时，如在遗传性大血小板减少症中，血小板压积（PCT）可提供重要的临床信息。
我们回顾性比较了4组犬的血小板计数和PCT与一般参考区间：患有非造血系统疾病的查理士王小猎犬（CKCS）和灵缇犬，在3年期间分别送至明尼苏达大学（使用Advia 2120检测）和奥本大学教学医院（使用Advia 120检测）。
确定了犬PCT的参考区间为0.129 - 0.403%。4个样本组中没有一组低于血小板压积参考区间的个体显著增多。对于血小板计数，只有2个CKCS组低于参考区间的个体比预期显著增多。
在临床环境中，使用Advia 120/2120测定的PCT似乎可避免高估患病CKCS的低血小板总量。相比之下，在评估患病灵缇犬的血小板总量时，PCT与血小板计数表现相似。在其他与平均血小板体积增加相关的情况下，应考虑评估PCT。
