Ravindran Resmi, Krishnan Viswanathan V, Dhawan Rajeev, Wunderlich Michelle L, Lerche Nicholas W, Flynn Joanne L, Luciw Paul A, Khan Imran H
Center for Comparative Medicine, University of California, Davis, CA, USA.
J Med Primatol. 2014 Apr;43(2):59-71. doi: 10.1111/jmp.12097. Epub 2014 Jan 22.
Tuberculosis (TB) in non-human primates (NHPs) is highly contagious, requiring efficient identification of animals infected with Mycobacterium tuberculosis. Tuberculin skin test is usually used but lacks desirable sensitivity/specificity and efficiency.
We aimed to develop an immunoassay for plasma antibodies against M. tuberculosis. A key challenge is that not all infected animals contain antibodies against the same M. tuberculosis antigen. Therefore, a multiplex panel of 28 antigens (Luminex(®) -Platform) was developed.
Data revealed antibodies against eight antigens (Rv3875, Rv3875-Rv3874 fusion, Rv3874, Rv0934, Rv3881, Rv1886c, Rv2031, Rv3841) in experimentally infected (M. tuberculosis strains: Erdman and H37Rv) NHPs (rhesus and cynomolgus macaques). In a naturally acquired M. tuberculosis infection, rhesus macaques (n = 15) with lung TB pathology (n = 10) contained antibodies to five additional antigens (Rv0831, Rv2220, Rv0054, Rv1099, and Rv0129c).
Results suggest that this user-friendly and easily implementable multiplex panel, containing 13 M. tuberculosis antigens, may provide a high-throughput alternative for NHP TB screening.
非人灵长类动物（NHPs）的结核病具有高度传染性，需要高效识别感染结核分枝杆菌的动物。通常使用结核菌素皮肤试验，但缺乏理想的敏感性/特异性和效率。
我们旨在开发一种针对结核分枝杆菌血浆抗体的免疫测定法。一个关键挑战是并非所有受感染动物都含有针对相同结核分枝杆菌抗原的抗体。因此，开发了一个包含28种抗原的多重检测板（Luminex®平台）。
数据显示，在实验感染（结核分枝杆菌菌株：埃尔德曼和H37Rv）的NHPs（恒河猴和食蟹猴）中存在针对八种抗原（Rv3875、Rv3875-Rv3874融合体、Rv3874、Rv0934、Rv3881、Rv1886c、Rv2031、Rv3841）的抗体。在自然获得的结核分枝杆菌感染中，患有肺结核病理（n = 10）的恒河猴（n = 15）含有针对另外五种抗原（Rv0831、Rv2220、Rv0054、Rv1099和Rv0129c）的抗体。
结果表明，这个包含13种结核分枝杆菌抗原的用户友好且易于实施 的多重检测板，可能为NHPs结核病筛查提供一种高通量替代方法。
