Zhang Yiqing, Zhou Lingling, Zhang Yongji, Tan Chaoqun
Key Laboratory of Yangtze River Water Environment, Ministry of Education (Tongji University), Shanghai, China.
Photochem Photobiol. 2014 May-Jun;90(3):609-14. doi: 10.1111/php.12210. Epub 2014 Jan 21.
This study aims at comparing the inactivation of Bacillus subtilis spores by various combinations of UV treatment and hydrogen peroxide (H2O2) addition. The combinations included sequential (UV-H2O2, H2O2-UV) and simultaneous (UV/H2O2) processes. Results showed that B. subtilis spores achieved a certain inactivation effect through UV treatment. However, hardly any inactivation effect by H2O2 alone was observed. H2O2 had a significant synergetic effect when combined with UV treatment, while high irradiance and H2O2 concentration both favored the reaction. When treated with 0.60 mm H2O2 and 113.0 μW/cm(2) UV irradiance for 6 min, the simultaneous UV/H2O2 treatment showed significantly improved disinfection effect (4.13 log) compared to that of UV-H2O2 (3.03 log) and H2O2-UV (2.88 log). The relationship between the inactivation effect and the exposure time followed a typical pseudo-first-order kinetics model. The pseudo-first-order rate constants were 0.478, 0.447 and 0.634 min(-1), for the UV-H2O2, H2O2-UV and UV/H2O2 processes, respectively, further confirming the optimal disinfection effect of the UV/H2O2 process. The disinfection could be ascribed to the OH radicals, as verified by the level of para-chlorobenzoic acid (pCBA).
本研究旨在比较紫外线处理和添加过氧化氢（H₂O₂）的各种组合对枯草芽孢杆菌孢子的灭活效果。这些组合包括顺序处理（紫外线-H₂O₂、H₂O₂-紫外线）和同时处理（紫外线/H₂O₂）过程。结果表明，枯草芽孢杆菌孢子通过紫外线处理可达到一定的灭活效果。然而，单独使用H₂O₂几乎未观察到灭活效果。H₂O₂与紫外线处理结合时具有显著的协同效应，而高辐照度和H₂O₂浓度均有利于该反应。当用0.60毫米H₂O₂和113.0微瓦/平方厘米的紫外线辐照度处理6分钟时，与紫外线-H₂O₂（3.03对数）和H₂O₂-紫外线（2.88对数）相比，同时进行的紫外线/H₂O₂处理显示出显著提高的消毒效果（4.13对数）。灭活效果与暴露时间之间的关系遵循典型的伪一级动力学模型。对于紫外线-H₂O₂、H₂O₂-紫外线和紫外线/H₂O₂过程，伪一级速率常数分别为0.478、0.447和0.634分钟⁻¹，进一步证实了紫外线/H₂O₂过程的最佳消毒效果。如对氯苯甲酸（pCBA）水平所验证的，消毒作用可归因于羟基自由基。
