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利用紫外线/过氧化氢高级氧化法灭活大肠杆菌、枯草芽孢杆菌孢子以及MS2、T4和T7噬菌体

Inactivation of E. coli, B. subtilis spores, and MS2, T4, and T7 phage using UV/H2O2 advanced oxidation.

作者信息

Mamane Hadas, Shemer Hilla, Linden Karl G

机构信息

School of Mechanical Engineering and Porter School of Environmental Studies, Tel-Aviv University, Tel-Aviv 69978, Israel.

出版信息

J Hazard Mater. 2007 Jul 31;146(3):479-86. doi: 10.1016/j.jhazmat.2007.04.050. Epub 2007 Apr 20.

Abstract

The goal of this study was to evaluate the potential of an advanced oxidation process (AOP) for microbiocidal and virucidal inactivation. The viruses chosen for this study were bacteriophage MS2, T4, and T7. In addition, Bacillus subtilis spores and Escherichia coli were studied. By using H(2)O(2) in the presence of filtered ultraviolet (UV) irradiation (UV/H(2)O(2)) to generate wavelengths above 295nm, the direct UV photolysis disinfection mechanism was minimized, while disinfection by H(2)O(2) was also negligible. Virus T4 and E. coli in phosphate buffered saline (PBS) were sensitive to >295nm filtered UV irradiation (without H(2)O(2)), while MS2 was very resistant. Addition of H(2)O(2) at 25mg/l in the presence of filtered UV irradiation over a 15min reaction time did not result in any additional disinfection of virus T4, while an additional one log inactivation for T7 and 2.5 logs for MS2 were obtained. With E. coli, only a slight additional effect was observed when H(2)O(2) was added. B. subtilis spores did not show any inactivation at any of the conditions used in this study. The OH radical exposure (CT value) was calculated to present the relationship between the hydroxyl radical dose and microbial inactivation.

摘要

本研究的目的是评估一种高级氧化工艺(AOP)用于杀菌和灭病毒的潜力。本研究选用的病毒是噬菌体MS2、T4和T7。此外,还对枯草芽孢杆菌孢子和大肠杆菌进行了研究。通过在过滤紫外线(UV)照射(UV/H₂O₂)存在的情况下使用H₂O₂来产生波长大于295nm的光,直接UV光解消毒机制被最小化,同时H₂O₂的消毒作用也可忽略不计。磷酸盐缓冲盐水(PBS)中的病毒T4和大肠杆菌对大于295nm的过滤紫外线照射(无H₂O₂)敏感,而MS2则具有很强的抗性。在15分钟的反应时间内,在过滤紫外线照射存在的情况下添加25mg/l的H₂O₂并没有导致病毒T4的任何额外消毒效果,而T7额外灭活了一个对数,MS2额外灭活了2.5个对数。对于大肠杆菌,添加H₂O₂时仅观察到轻微的额外效果。枯草芽孢杆菌孢子在本研究使用的任何条件下均未显示出任何灭活现象。计算了羟基自由基暴露量(CT值)以呈现羟基自由基剂量与微生物灭活之间的关系。

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