Department of Pharmacology, Aichi Medical University Graduate School of Medicine, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan; Department of Pharmacy, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.
Department of Pharmacology, Aichi Medical University Graduate School of Medicine, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.
Auton Neurosci. 2014 Apr;181:49-54. doi: 10.1016/j.autneu.2013.12.013. Epub 2014 Jan 8.
The hypothalamic paraventricular nucleus (PVN) is one of the most important autonomic control centers in the brain. Several kinds of prostanoids, such as prostaglandin (PG) E2, are considered to act in the PVN as mediators of autonomic responses. In the present study, we used liquid chromatography ion trap tandem mass spectrometry (LC-ITMS(n)) to simultaneously quantify four prostanoids, thromboxane (Tx) B2, PGE2, PGD2 and 15-deoxy-∆(12,14) (15d)-PGJ2 in PVN microdialysates from urethane-anesthetized rats. The quantification limits were estimated to be 0.05ng/mL for TxB2, 0.025ng/mL for PGE2, 0.1ng/mL for PGD2, and 0.5ng/mL for 15d-PGJ2. The RSD% obtained from all prostanoids was <15%, indicating an acceptable level of reproducibility. LC-ITMS(n) analysis of rat PVN microdialysates revealed that TxA2 may play an important role in adrenomedullary outflow evoked by centrally administered N-methyl-d-aspartate, corticotrophin-releasing factor and glucagon-like peptide-1. This is the first study to use LC-ITMS(n) to analyze prostanoid levels in rat PVN microdialysates. This LC-ITMS(n) method will be useful for investigating the potential involvement of prostanoids in brain function.
下丘脑室旁核（PVN）是大脑中最重要的自主控制中心之一。几种前列腺素，如前列腺素（PG）E2，被认为在 PVN 中作为自主反应的介质起作用。在本研究中，我们使用液相色谱-离子阱串联质谱（LC-ITMS(n)）同时定量测定戊巴比妥钠麻醉大鼠 PVN 微透析液中的四种前列腺素，血栓素（Tx）B2、PGE2、PGD2 和 15-脱氧-∆(12,14)（15d）-PGJ2。定量限估计为 TxB2 为 0.05ng/mL，PGE2 为 0.025ng/mL，PGD2 为 0.1ng/mL，15d-PGJ2 为 0.5ng/mL。所有前列腺素的 RSD%均<15%，表明具有可接受的重现性。LC-ITMS(n)分析大鼠 PVN 微透析液表明，TxA2 可能在中枢给予 N-甲基-D-天冬氨酸、促肾上腺皮质释放因子和胰高血糖素样肽-1 引起的肾上腺髓质流出中发挥重要作用。这是首次使用 LC-ITMS(n)分析大鼠 PVN 微透析液中前列腺素水平的研究。这种 LC-ITMS(n)方法将有助于研究前列腺素在大脑功能中的潜在作用。
