甲状腺乳头状癌中hMLH1基因启动子的异常甲基化及其临床意义

[Aberrant methylation of hMLH1 gene promoter in papillary thyroid cancer and its clinical significance].

作者信息

Lu Xiao-xiao, Ge Ming-hua, Ling Zhi-qiang, Hu Si-si, Xu Jing, Zheng Chuan-ming, Tan Zhuo, Chen Chao

机构信息

Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China.

Email:

出版信息

Zhonghua Zhong Liu Za Zhi. 2013 Nov;35(11):833-6.

PMID:24447481

Abstract

OBJECTIVE

To investigate the aberrant promoter methylation of hMLH1 gene promoter and its clinical significance in papillary thyroid cancer (PTC).

METHODS

methylation of hMLH1 gene promoter in the cancer tissue and matched tumor-adjacent normal tissue of 152 PTC patients were detected by real-time methylation specific PCR (qMSP). The relationship between the methylation of hMLH1 gene promoter and clinicopathological features was analyzed.

RESULTS

The methylation rate of hMLH1 gene promoter in cancer tissues was 37.5% (57/152), of which 33 cases were totally methylated and 24 cases were partially methylated. The methylation rate of adjacent normal tissues was 5.3% (8/152)(all were partially methylated). The methylation rate of PTC tissues was significantly higher than that in the tumor-adjacent normal tissue (P < 0.01). The promoter methylation of hMLH1 gene in PTC was significantly correlated with age, size and number of the primary lesion, local invasion, T stage and lymph node metastasis (P < 0.05) , but not correlated with gender and clinical stage (P > 0.05).

CONCLUSION

Promoter methylation of hMLH1 gene is a common molecular event in PTC tissue, and it is significantly correlated with the progression of PTC.

摘要

目的

探讨人错配修复蛋白1（hMLH1）基因启动子异常甲基化及其在甲状腺乳头状癌（PTC）中的临床意义。

方法

采用实时甲基化特异性PCR（qMSP）检测152例PTC患者癌组织及配对癌旁正常组织中hMLH1基因启动子的甲基化情况，并分析hMLH1基因启动子甲基化与临床病理特征的关系。

结果

癌组织中hMLH1基因启动子甲基化率为37.5%（57/152），其中完全甲基化33例，部分甲基化24例；癌旁正常组织甲基化率为5.3%（8/152）（均为部分甲基化）。PTC组织甲基化率显著高于癌旁正常组织（P<0.01）。PTC中hMLH1基因启动子甲基化与年龄、原发灶大小及数量、局部侵犯、T分期和淋巴结转移显著相关（P<0.05），与性别和临床分期无关（P>0.05）。