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MLH1启动子甲基化对头颈部鳞状细胞癌诊断价值的系统评价与Meta分析方法

A systematic review and meta-analysis approach on diagnostic value of MLH1 promoter methylation for head and neck squamous cell carcinoma.

作者信息

Li Qun, Hong Jinjiong, Shen Zhisen, Deng Hongxia, Shen Yi, Wu Zhenhua, Zhou Chongchang

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital (Lihuili Hospital of Ningbo University).

Department of Hand Surgery, Ningbo 6th Hospital, Ningbo University.

出版信息

Medicine (Baltimore). 2019 Oct;98(43):e17651. doi: 10.1097/MD.0000000000017651.

DOI:10.1097/MD.0000000000017651
PMID:31651887
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6824735/
Abstract

BACKGROUND

Head and neck squamous cell carcinoma (HNSCC) is the leading histological type among head and neck cancers. Several studies have explored an association between aberrant methylation of MutL homolog-1 (MLH1) promoter and HNSCC risk. We aimed to explore the associations between MLH1 promoter methylation and HNSCC by using a meta-analysis.

METHODS

Systematic literature search was conducted among PubMed, Google Scholar, Web of Science, and China National Knowledge Infrastructure, and Wanfang databases to retrieve relevant articles published up to June 30, 2018. A total of 12 studies were included in this meta-analysis (including 717 HNSCC and 609 controls).

RESULTS

The results demonstrated that MLH1 promoter methylation was notably higher in patients with HNSCC than in controls (odds ratios [ORs] = 2.52, 95% confidence intervals [CIs] = 1.33-4.79). Besides, MLH1 promoter methylation was not associated with tumor stage, lymph node status, smoking behavior, age, clinical stage, gender, and differentiation grade (all P > .05). The pooled sensitivity and specificity rates of MLH1 methylation for HNSCC were 0.23 (95% CI = 0.12-0.38) and 0.95 (95% CI, 0.82-0.99), respectively. The area under the receiver operating characteristic (ROC) curve was presented as 0.64 (95% CI = 0.60-0.68).

CONCLUSION

The results of this meta-analysis suggested that hypermethylation of MLH1 promoter was associated with HNSCC. Methylated MLH1 could be a potential diagnostic biomarker for diagnose of HNSCC.

摘要

背景

头颈部鳞状细胞癌（HNSCC）是头颈部癌症中最主要的组织学类型。多项研究探讨了MutL同源蛋白1（MLH1）启动子异常甲基化与HNSCC风险之间的关联。我们旨在通过荟萃分析探讨MLH1启动子甲基化与HNSCC之间的关联。

方法

在PubMed、谷歌学术、科学网和中国知网以及万方数据库中进行系统的文献检索，以检索截至2018年6月30日发表的相关文章。本荟萃分析共纳入12项研究（包括717例HNSCC患者和609例对照）。

结果

结果表明，HNSCC患者中MLH1启动子甲基化显著高于对照组（优势比[ORs]=2.52，95%置信区间[CIs]=1.33-4.79）。此外，MLH1启动子甲基化与肿瘤分期、淋巴结状态、吸烟行为、年龄、临床分期、性别和分化程度均无关（所有P>.05）。MLH1甲基化对HNSCC的合并敏感度和特异度分别为0.23（95%CI=0.12-0.38）和0.95（95%CI，0.82-0.99）。受试者工作特征（ROC）曲线下面积为0.64（95%CI=0.60-0.68）。

结论

本荟萃分析结果提示，MLH1启动子高甲基化与HNSCC有关。甲基化的MLH1可能是诊断HNSCC的潜在生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/ebb135b2584b/medi-98-e17651-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/9f8f4b160e7a/medi-98-e17651-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/334b7c1150e4/medi-98-e17651-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/dfd96b0678c3/medi-98-e17651-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/bff09c08df15/medi-98-e17651-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/ecda80c57298/medi-98-e17651-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/ebb135b2584b/medi-98-e17651-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/9f8f4b160e7a/medi-98-e17651-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/334b7c1150e4/medi-98-e17651-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/dfd96b0678c3/medi-98-e17651-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/bff09c08df15/medi-98-e17651-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/ecda80c57298/medi-98-e17651-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb3/6824735/ebb135b2584b/medi-98-e17651-g010.jpg

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