Wang Yicun, Su Quanping, Dong Shuai, Shi Hongxi, Gao Xiang, Wang Li
Institute of Genetics and Cytology; School of Life Sciences; Northeast Normal University; Changchun City, Jilin Province, PR China.
Hum Vaccin Immunother. 2014;10(4):1057-63. doi: 10.4161/hv.27714. Epub 2014 Jan 21.
The polymorphic fungus Candida albicans (C. albicans) can live as an aggressive pathogen and cause many diseases in hosts, for which no effective vaccine exists. The secreted aspartyl proteinase 2 (Sap2) plays a protective role in systemically infected BALB/c mice. Protective cellular immune responses can be preferentially induced when antigens are displayed on small particles. Therefore, the emphasis is placed on developing new phage vaccine to inhibit C. albicans infection. In this study, the ability of the hybrid phage displaying the epitope SLAQVKYTSASSI and recombinant protein of Sap2 (rSap2) for inducing immune protective responses against C. albicans infection was evaluated by lymphoproliferative assay, to gather cytokine and antibody measurements in BALB/c mice. Our results showed that, strong cellular and humoral immune responses were induced in a mouse model immunized with hybrid phage or rSap2. Furthermore, the protection against lethal challenge with C. albicans was observed in mice vaccinated hybrid phage without adjuvant. These findings demonstrate that the hybrid phage displaying the epitope SLAQVKYTSASSI might be a potential vaccine against C. albicans infections.
多态性真菌白色念珠菌（白色念珠菌）可作为一种侵袭性病原体存在，并在宿主体内引发多种疾病，目前尚无有效的疫苗。分泌天冬氨酸蛋白酶2（Sap2）在全身感染的BALB/c小鼠中发挥保护作用。当抗原呈递在小颗粒上时，可优先诱导保护性细胞免疫反应。因此，重点在于开发新型噬菌体疫苗以抑制白色念珠菌感染。在本研究中，通过淋巴细胞增殖试验评估了展示表位SLAQVKYTSASSI的杂交噬菌体和Sap2重组蛋白（rSap2）诱导针对白色念珠菌感染的免疫保护反应的能力，以收集BALB/c小鼠的细胞因子和抗体测量数据。我们的结果表明，用杂交噬菌体或rSap2免疫的小鼠模型中诱导了强烈的细胞免疫和体液免疫反应。此外，在未使用佐剂接种杂交噬菌体的小鼠中观察到了对白色念珠菌致死性攻击的保护作用。这些发现表明，展示表位SLAQVKYTSASSI的杂交噬菌体可能是一种针对白色念珠菌感染的潜在疫苗。
