Samuels B L, Rosner M C, Giometti C S, Golomb H M, Brownstein B H
Joint Section of Hematology/Oncology, University of Chicago, IL 60637.
Leukemia. 1987 Apr;1(4):365-9.
We have investigated the direct effects of interferon (IFN) on hairy cells (HCs) isolated from patients with hairy cell leukemia using one- and two-dimensional gel electrophoresis. We have previously characterized the induction of synthesis of 10-16 specific proteins by IFN-alpha 2b in HCs, as analyzed by one-dimensional electrophoresis. By two-dimensional electrophoresis, we have now confirmed this induction and shown that the synthesis of the same number of specific proteins is down-regulated in HCs exposed to IFN-alpha 2b. When compared to HCs, fewer proteins are induced by IFN-alpha 2b in other normal, or neoplastic, lymphoid cells. We also report that protein induction occurs in HCs exposed in vivo to IFN-alpha 2b. We have demonstrated the presence of tubuloreticular structures in the cytoplasm of HCs exposed to IFN-alpha 2b in vitro, using transmission electron microscopy. We now report that these too are seen in HCs exposed to IFN in vivo during therapy. We investigated the effects of IFN-gamma on HCs and found that it also induces specific proteins. The pattern of induced proteins is distinctly different after IFN-gamma exposure in vitro. The fact that such induction occurs suggests that HCs possess also a receptor for IFN-gamma. These results demonstrate that there are direct effects of IFN-alpha on HCs and that such direct effects might be important in the antitumor activity of IFN-alpha in hairy cell leukemia.
我们使用一维及二维凝胶电泳技术，研究了干扰素（IFN）对从毛细胞白血病患者分离出的毛细胞（HCs）的直接作用。我们之前通过一维电泳分析，已对α-2b干扰素在毛细胞中诱导合成10 - 16种特异性蛋白质进行了表征。通过二维电泳，我们现已证实了这种诱导作用，并表明在暴露于α-2b干扰素的毛细胞中，相同数量的特异性蛋白质合成被下调。与毛细胞相比，α-2b干扰素在其他正常或肿瘤性淋巴细胞中诱导的蛋白质较少。我们还报告了在体内暴露于α-2b干扰素的毛细胞中发生了蛋白质诱导现象。我们利用透射电子显微镜，证明了在体外暴露于α-2b干扰素的毛细胞胞质中存在管状网状结构。我们现在报告，在治疗期间体内暴露于干扰素的毛细胞中也可见到这些结构。我们研究了γ干扰素对毛细胞的作用，发现它也能诱导特异性蛋白质。体外暴露于γ干扰素后，诱导蛋白质的模式明显不同。这种诱导作用的发生表明毛细胞也拥有γ干扰素受体。这些结果表明，α干扰素对毛细胞有直接作用，且这种直接作用可能在α干扰素对毛细胞白血病的抗肿瘤活性中起重要作用。
