Griffiths S D, Cawley J C
Department of Haematology, University of Liverpool, Royal Liverpool Hospital, U.K.
Leukemia. 1987 Apr;1(4):372-6.
The natural killer (NK) activity of peripheral blood mononuclear cells against K562 targets was variable in 10 untreated patients with hairy cell leukemia and was inversely related to the number of hairy cells (HCs) present. After therapy with alpha-interferon (IFN-alpha) NK activity in vitro was equivalent to that of normal controls. It is suggested that the low activity often seen before treatment is attributable to dilution of NK cells by large numbers of inactive HCs and that this diluting effect is reduced as HCs disappear from the blood during IFN-alpha treatment. HCs was consistently resistant to NK lysis by normal or hairy cell leukemia allogeneic and autologous mononuclear cells, despite whether effector or target cells had been pretreated with IFN-alpha. Cold-target inhibition and direct binding experiments showed that HCs do not bind to NK effectors. It is therefore concluded that NK cells play no direct role in the progressive disappearance of HCs seen in patients receiving IFN-alpha.
10例未经治疗的毛细胞白血病患者外周血单个核细胞对K562靶细胞的自然杀伤（NK）活性存在差异，且与毛细胞（HC）数量呈负相关。用α干扰素（IFN-α）治疗后，体外NK活性与正常对照相当。提示治疗前常见的低活性是由于大量无活性的HC稀释了NK细胞，并且在IFN-α治疗期间随着HC从血液中消失，这种稀释作用减弱。HC对正常或毛细胞白血病的同种异体和自体单个核细胞的NK裂解始终具有抗性，无论效应细胞或靶细胞是否用IFN-α预处理。冷靶抑制和直接结合实验表明HC不与NK效应细胞结合。因此得出结论，NK细胞在接受IFN-α治疗的患者中HC的逐渐消失过程中不发挥直接作用。
