AIT Austrian Institute of Technology GmbH, Health & Environment Department, Molecular Diagnostics, 1220 Vienna, Austria.
Chem Commun (Camb). 2014 Mar 7;50(19):2424-7. doi: 10.1039/c3cc48640k. Epub 2014 Jan 22.
Biotin- and iminobiotin-bonded surfaces obtained by thiol-ene chemistry and subsequent modification with polyamines were characterized with respect to streptavidin-binding capacity and reversibility for photonic biosensing using X-ray photoelectron spectroscopy and Mach-Zehnder-interferometric sensors. The streptavidin-iminobiotin system was exploited for reversible multilayer deposition and determination of affinity constants on each layer.
通过硫醇-烯化学反应获得的生物素和亚氨基生物素键合表面，并随后用多胺进行修饰，通过 X 射线光电子能谱和马赫-曾德尔干涉传感器对其与链霉亲和素的结合能力和光学生物传感的可逆性进行了表征。利用链霉亲和素-亚氨基生物素体系实现了可逆多层沉积，并确定了每层的亲和常数。
