由牛血清白蛋白和β-环糊精键合硅胶制备复合手性固定相

Development of a composite chiral stationary phase from BSA and β-cyclodextrin-bonded silica.

作者信息

Yao Bixia, Yang Xinmei, Guo Lizhen, Kang Shanshan, Weng Wen

机构信息

Department of Chemistry and Environmental Science, Minnan Normal University, 36 Xianqian Straight Street, Xiangcheng District, Zhangzhou 363000, China Key Laboratory for Analytical Science of Fujian Province University, Zhangzhou 363000, China.

出版信息

J Chromatogr Sci. 2014 Nov-Dec;52(10):1233-8. doi: 10.1093/chromsci/bmt203. Epub 2014 Jan 20.

DOI:10.1093/chromsci/bmt203

PMID:24448668

Abstract

A composite chiral stationary phase (CSP) derived from bovine serum albumin (BSA) and β-cyclodextrin (CD)-bonded silica was prepared. 2,4,6-Trichloro-1,3,5-triazine was used as a cross-linker. The obtained CSP was applied to the enantioseparation of tryptophan, hydrobenzoin, phenylalanine and mandelic acid. The influences of eluent pH value, organic modifier and column temperature on the retention and enantioseparation were discussed. Tryptophan and hydrobenzoin achieved excellent resolution on the composite CSP. For tryptophan, the highest selectivity, 2.79, was achieved with 1% of methanol at pH 8.0. For hydrobenzoin, the selectivity could reach 1.42. The chromatographic results were compared with that on β-CD-bonded or BSA-immobilized CSP.

摘要

制备了一种由牛血清白蛋白（BSA）和β-环糊精（CD）键合硅胶衍生的复合手性固定相（CSP）。使用2,4,6-三氯-1,3,5-三嗪作为交联剂。将所得的CSP应用于色氨酸、氢化苯偶姻、苯丙氨酸和扁桃酸的对映体分离。讨论了洗脱液pH值、有机改性剂和柱温对保留和对映体分离的影响。色氨酸和氢化苯偶姻在复合CSP上实现了优异的分离度。对于色氨酸，在pH 8.0下使用1%甲醇时，实现了最高选择性2.79。对于氢化苯偶姻，选择性可达1.42。将色谱结果与β-CD键合或BSA固定的CSP上的结果进行了比较。