Anglade P, Michel C, Rozé C
INSERM U 239, Faculté de Médecine X, Bichat, Paris, France.
Pancreas. 1987;2(5):568-77. doi: 10.1097/00006676-198709000-00013.
The effect of surgical celiac and superior mesenteric ganglionectomy (GGX) on intrinsic pancreatic innervation was studied in male Wistar rats. Neurons were stained in the pancreas of control and ganglionectomized rats with histochemical methods evidencing the acetylcholinesterase (AChE) activity and the induced fluorescence of catecholamines. Two segments of the head of the pancreas ("duodenal" and "splenic" segments) were studied. The surface density of neuronal cell bodies was larger in the splenic segment than in the duodenal segment of the head of the pancreas (p less than 0.01), both in control and in ganglionectomized rats. The surface density of the neuronal cell bodies and the intensity of AChE staining were not significantly different in control and in ganglionectomized rats. Most AChE-positive fibers survived after ganglionectomy; only some of them disappeared. These disappearing fibers corresponded to the thinnest fiber bundles found in the pancreatic tissue of control rats. Only 10% of catecholamine fibers remained after ganglionectomy. Some of the remaining fibers may run through direct duodenopancreatic pathways.
在雄性Wistar大鼠中研究了手术切除腹腔神经节和肠系膜上神经节（GGX）对胰腺内在神经支配的影响。采用组织化学方法对对照大鼠和神经节切除大鼠的胰腺神经元进行染色，以显示乙酰胆碱酯酶（AChE）活性和儿茶酚胺诱导荧光。研究了胰腺头部的两个节段（“十二指肠”和“脾”节段）。无论是对照大鼠还是神经节切除大鼠，胰腺头部脾节段神经元细胞体的表面密度均大于十二指肠节段（p<0.01）。对照大鼠和神经节切除大鼠的神经元细胞体表面密度和AChE染色强度无显著差异。神经节切除后，大多数AChE阳性纤维存活；只有一些消失。这些消失的纤维对应于对照大鼠胰腺组织中最细的纤维束。神经节切除后仅10%的儿茶酚胺纤维留存。部分留存纤维可能通过直接的十二指肠-胰腺通路走行。
