Hiramatsu K, Watanabe T, Fujioka T
Department of Veterinary Anatomy, Faculty of Agriculture, Nagoya University, Japan.
Arch Histol Cytol. 1988 May;51(2):159-68. doi: 10.1679/aohc.51.159.
The distribution of acetylcholinesterase (AChE)-positive nerve fibers in the chicken pancreas was investigated with histochemical methods at the light and electron microscopic level. AChE-positive nerve bundles were found to run along the pancreaticoduodenal artery and their branches proceed into interlobular connective tissue, form a plexus around the interlobular secretory ducts and small arteries, and penetrate the exocrine parenchyma. Intrapancreatic ganglia showing a strong AChE activity were detected within the interlobular connective tissue or between acini. The exocrine pancreas was richly innervated with AChE-positive terminals which contained a few large dense-cored vesicles (about 100 nm in diameter) and many small clear vesicles (about 50 nm in diameter). Such terminals made contact with intercalated ductular cells and the smooth muscles of larger blood vessels. The endocrine pancreas was supplied with fewer nerves than the exocrine pancreas. A different distribution of AChE-positive fibers was noticed between A- and B-islets which were distinguished immunohistochemically. B- and D-cells were richly innervated by AChE-positive nerves, whereas A-cells, only poorly. These observations make clear that the cholinergic system relates to the regulation of both exocrine and endocrine tissues, except A-cells, in the chicken pancreas.
采用组织化学方法，在光镜和电镜水平上研究了鸡胰腺中乙酰胆碱酯酶（AChE）阳性神经纤维的分布。发现AChE阳性神经束沿胰十二指肠动脉走行，其分支进入小叶间结缔组织，在小叶间分泌导管和小动脉周围形成神经丛，并穿透外分泌实质。在小叶间结缔组织内或腺泡之间检测到具有强AChE活性的胰腺内神经节。外分泌胰腺有丰富的AChE阳性终末神经支配，这些终末含有一些大的致密核心囊泡（直径约100nm）和许多小的清亮囊泡（直径约50nm）。这些终末与闰管细胞和较大血管的平滑肌接触。内分泌胰腺的神经支配比外分泌胰腺少。在通过免疫组织化学区分的A胰岛和B胰岛之间，观察到AChE阳性纤维的分布不同。B细胞和D细胞有丰富的AChE阳性神经支配，而A细胞的神经支配较少。这些观察结果表明，胆碱能系统与鸡胰腺中外分泌和内分泌组织（A细胞除外）的调节有关。
