Choudhary Shanti, Zieger Ulrike, Sharma Ravindra N, Chikweto Alfred, Tiwari Keshaw P, Ferreira Leandra R, Oliveira Solange, Barkley Lovell J, Verma Shiv Kumar, Kwok Oliver C H, Su Chunlei, Dubey J P
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705, USA.
Anatomy, Physiology, Pharmacology, Pathobiology Academic Programs School of Veterinary Medicine, St. George's University, Grenada, West Indies.
J Zoo Wildl Med. 2013 Dec;44(4):1127-30. doi: 10.1638/2013-0129.1.
Little is known of the genetic diversity and epidemiology of Toxoplasma gondii infection in wildlife in Caribbean Islands. The prevalence and genetic diversity of T. gondii in mongooses (Herpestes auropunctatus) was investigated. During 2011 and 2012, 91 mongooses were trapped in different parts of Grenada, bled, euthanized, and examined at necropsy. Antibodies to T. gondii were found in 27 mongooses tested by the modified agglutination test (cut-off titer 25). Muscles (heart, tongue, neck) of 25 of the seropositive mongooses were bioassayed for T. gondii infection in mice. Viable T. gondii was isolated by bioassay in mice from four mongooses with MAT titers of 1:50 in two, 1:200 for one, and 1:400 for one mongoose. The four T. gondii isolates were further propagated in cell culture. Strain typing of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed one isolate belongs to the Type III (ToxoDB #2) lineage, two to ToxoDB#7 lineage, and one to the ToxoDB #216 lineage. This is the first report of T. gondii isolation and genotyping in H. auropunctatus worldwide.
关于加勒比群岛野生动物中弓形虫感染的遗传多样性和流行病学，人们了解甚少。本研究调查了猫鼬（长尾鼬）中弓形虫的流行情况和遗传多样性。在2011年至2012年期间，在格林纳达的不同地区捕获了91只猫鼬，采集血液后实施安乐死，并在尸检时进行检查。通过改良凝集试验（临界滴度25）检测的91只猫鼬中，有27只检测出抗弓形虫抗体。对25只血清阳性猫鼬的肌肉（心脏、舌头、颈部）进行生物测定，以检测小鼠是否感染弓形虫。通过生物测定，在小鼠中从4只猫鼬分离出活的弓形虫，其中2只猫鼬的改良凝集试验滴度为1:50，1只猫鼬为1:200，1只猫鼬为1:400。这4株弓形虫分离株在细胞培养中进一步传代培养。使用10种PCR-限制性片段长度多态性（RFLP）标记SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico，对从细胞培养速殖子中提取的弓形虫DNA进行菌株分型，结果显示1株分离株属于III型（ToxoDB #2）谱系，2株属于ToxoDB#7谱系，1株属于ToxoDB #216谱系。这是全球范围内首次关于长尾鼬中弓形虫分离和基因分型的报告。
