刚地弓形虫克隆 III 型是在格林纳达猪中鉴定到的主要基因型。

Toxoplasma gondii clonal type III is the dominant genotype identified in Grenadian pigs.

机构信息

Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada.

Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA.

出版信息

Vet Med Sci. 2022 Sep;8(5):2005-2011. doi: 10.1002/vms3.870. Epub 2022 Jul 9.

DOI:10.1002/vms3.870

PMID:35809056

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9514473/

Abstract

BACKGROUND

Toxoplasma gondii is a widespread zoonotic protozoan parasite capable of infecting all warm-blooded animals. Although the genotypes of T. gondii in pigs have been reported worldwide, there is no information on the genotypes and diversity of T. gondii in pigs in Grenada, West Indies.

OBJECTIVES

The aims of the present study were to isolate, genotype and determine the diversity of T. gondii genotypes in pigs.

METHODS

We carried out a modified agglutination test (MAT) on blood from 149 pig hearts collected from a local meat market. Myocardial tissue homogenate from pigs that tested positive for T. gondii was homogenized and inoculated into mice for isolation of the parasite. We collected mouse tissues and extracted DNA for genotyping based on 11 polymerase chain reaction-restriction fragment length polymorphism markers (SAG1, SAG2, alt. SAG2, SAG 3, BTUB, GRA6, L358, PK1, C22-8, C 29-2 and Apico).

RESULTS

Out of the 149 pig hearts, 31 (20.8%) tested positive for T. gondii on MAT. Bioassays in mice yielded 12 isolates designated TgpgGr1 to TgpgGr12. Molecular characterisation of T. gondii revealed four genotypes as follows: ToxoDB #2-clonal type III (seven isolates); ToxoDB #7 (three isolates); ToxoDB #13 (one isolate); ToxoDB #30 (1 isolate). Overall, ToxoDB #2 was the most common (58%). Toxo database (DB) # 13, which causes interstitial pneumonia in affected mice, has also been reported.

CONCLUSION

The genetic diversity of T. gondii in pigs in Grenada is lower than that in other surrounding Caribbean areas.

摘要

背景

刚地弓形虫是一种广泛传播的动物源性原虫寄生虫，能够感染所有温血动物。虽然已在全球范围内报道了猪弓形虫的基因型，但关于格林纳达（西印度群岛中的一个国家）猪弓形虫的基因型和多样性尚无信息。

目的

本研究旨在分离、基因分型并确定猪弓形虫基因型的多样性。

方法

我们对从当地肉市场采集的 149 份猪心血液进行改良凝集试验（MAT）。对弓形虫检测呈阳性的猪的心肌组织匀浆进行匀浆并接种于小鼠以分离寄生虫。我们采集小鼠组织并提取 DNA 进行基因分型，基于 11 个聚合酶链反应-限制性片段长度多态性标记（SAG1、SAG2、替代 SAG2、SAG3、BTUB、GRA6、L358、PK1、C22-8、C29-2 和 Apico）。

结果

在 149 份猪心中，31 份（20.8%）在 MAT 上检测出弓形虫阳性。在小鼠生物测定中获得了 12 个分离株，命名为 TgpgGr1 至 TgpgGr12。对弓形虫的分子特征分析显示有四种基因型：ToxoDB#2-克隆型 III（7 个分离株）；ToxoDB#7（3 个分离株）；ToxoDB#13（1 个分离株）；ToxoDB#30（1 个分离株）。总体而言，ToxoDB#2 最为常见（58%）。ToxoDB#13 也有报道，它会导致受感染小鼠发生间质性肺炎。