Walsh Thomas K
a Sustainable Agricultural Flagship, CSIRO Ecosystem Sciences , Canberra , Australia.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):167-8. doi: 10.3109/19401736.2013.878927. Epub 2014 Jan 22.
Australothis rubrescens is basal to the Helicoverpa lineage containing pests such as Helicoverpa armigera, H. assulta and H. gelotopoeon. An illumina library of DNA from A. rubrescens was constructed and shallow sequencing and assembly of the DNA was conducted. The complete mitochondrial genome was identified using similarity to the H. armigera mitochondrial genome. The mitochondrial genome of A. rubrescens is 15,382 bp in length. It contains 37 genes which are shared with the vast majority of animals: 13 protein-coding genes (PCGs), 2 ribosomal RNAs, 22 transfer RNAs and a non-coding AT-rich region (Table 1). As found in other Lepidopterans, the arrangement of all tRNAs of the A. rubrescens is identical to most insects. The complete mitochondrial genome of A. rubrescens will be an important tool in understanding the evolutionary history of the Heliothine moths.
澳洲红虫(Australothis rubrescens)位于棉铃虫谱系的基部,该谱系包含棉铃虫(Helicoverpa armigera)、烟青虫(H. assulta)和棉铃实夜蛾(H. gelotopoeon)等害虫。构建了来自澳洲红虫的DNA的Illumina文库,并对DNA进行了浅层测序和组装。利用与棉铃虫线粒体基因组的相似性鉴定出了完整的线粒体基因组。澳洲红虫的线粒体基因组长度为15382 bp。它包含37个与绝大多数动物共有的基因:13个蛋白质编码基因(PCGs)、2个核糖体RNA、22个转运RNA和一个富含AT的非编码区域(表1)。正如在其他鳞翅目昆虫中发现的那样,澳洲红虫所有tRNA的排列与大多数昆虫相同。澳洲红虫完整的线粒体基因组将成为理解实夜蛾进化历史的重要工具。