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果蛀蛾(林奈,1763年)(鳞翅目:夜蛾总科)的完整线粒体基因组序列

Complete mitochondrial genome sequence of fruit-piercing moth (Linnaeus, 1763) (Lepidoptera: Noctuoidea).

作者信息

Sivasankaran Kuppusamy, Mathew Pratheesh, Anand Sekar, Ceasar Stanislaus Antony, Mariapackiam Soosaimanikam, Ignacimuthu Savarimuthu

机构信息

Entomology Research Institute, Loyola College, University of Madras, Chennai 600 034, Tamil Nadu, India.

Centre for Plant Sciences, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, LS29JT, United Kingdom.

出版信息

Genom Data. 2017 Sep 28;14:66-81. doi: 10.1016/j.gdata.2017.09.004. eCollection 2017 Dec.

Abstract

The complete mitochondrial genome of the fruit piercing moth (Linnaeus, 1763) (Lepidoptera: Noctuoidea) was sequenced and characterized (Genbank Accession No: KY196412). The complete mitogenome is a circular molecule of 15,575 bp length, consisting of 13 protein-coding genes (PCGs), two ribosomal RNA genes ( and ), 22 transfer RNA (tRNA) genes and an A + T-rich region (D-loop). The nucleotide composition of the genome is highly A + T biased, accounting for 80.67% of nucleotides. All tRNAs have putative secondary structures that are characteristic of mitochondrial tRNA. Most of the PCGs were initiated by typical ATN codons. Five genes were initiated by unusual codons. gene was initiated by an unusual CGA codon and terminated by the typical stop codon GAA. Six genes ended with a single T. The A + T-rich region of 336 bp consisted of repetitive sequences, including two ATAGA motifs, a 19 bp poly-T stretch and three microsatellite-like regions ((TA), (TA) and two (TA)) Moreover, three large tandem (one 40 bp and two 25 bp) repeated elements were identified in A + T-rich region. Phylogenetic analysis using PCGs revealed that Superfamily Noctuoidea is a monophyletic group.

摘要

对果实穿孔蛾(林奈,1763年)(鳞翅目:夜蛾总科)的完整线粒体基因组进行了测序和特征分析(Genbank登录号:KY196412)。完整的线粒体基因组是一个长度为15575 bp的环状分子,由13个蛋白质编码基因(PCGs)、两个核糖体RNA基因( 和 )、22个转移RNA(tRNA)基因和一个富含A+T的区域(D环)组成。基因组的核苷酸组成高度偏向A+T,占核苷酸的80.67%。所有tRNA都具有推测的二级结构,这是线粒体tRNA的特征。大多数PCG由典型的ATN密码子起始。五个基因由不寻常的密码子起始。 基因由不寻常的CGA密码子起始,由典型的终止密码子GAA终止。六个基因以单个T结尾。富含A+T的区域为336 bp,由重复序列组成,包括两个ATAGA基序、一个19 bp的多聚T序列和三个微卫星样区域((TA)、(TA)和两个(TA))。此外,在富含A+T的区域鉴定出三个大的串联重复元件(一个40 bp和两个25 bp)。使用PCG进行的系统发育分析表明,夜蛾总科是一个单系类群。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a52/5633087/0b340f765931/gr1.jpg

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