Hunter J B, Hunter S M
MRC Experimental Embryology and Teratology Unit, Medical Research Council Laboratories, Carshalton, Surrey, United Kingdom.
Anal Biochem. 1987 Aug 1;164(2):430-3. doi: 10.1016/0003-2697(87)90515-x.
A simple assay for proteins in the low nanogram range is described. Proteins are bound to nitrocellulose paper in a band, stained with the commercially available colloidal gold stain AuroDye (a registered trademark of Jansen Life Sciences Products), and quantified by scanning densitometry: 1.25 to 20 ng of protein can be consistently measured. Variation in the staining intensity of different proteins is discussed. DNA, RNA, polyvinylpyrrolidone, Ficoll, and 6 M urea do not affect the assays, and samples from cellular material in a simple lysis buffer containing 0.1% of sodium dodecyl sulfate can be assayed.
本文描述了一种用于检测低纳克范围内蛋白质的简单方法。蛋白质以条带形式结合在硝酸纤维素纸上，用市售的胶体金染色剂AuroDye（扬森生命科学产品公司的注册商标）进行染色，并通过扫描密度测定法定量：可始终如一地检测到1.25至20纳克的蛋白质。文中讨论了不同蛋白质染色强度的差异。DNA、RNA、聚乙烯吡咯烷酮、聚蔗糖和6M尿素不影响该检测，并且可以对含有0.1%十二烷基硫酸钠的简单裂解缓冲液中细胞材料的样品进行检测。
