一种使用激光密度测定法的微量分析蛋白质测定法。
A microanalytical protein assay using laser densitometry.
作者信息
Sportsman J R, Elder J H
出版信息
Anal Biochem. 1984 Jun;139(2):298-300. doi: 10.1016/0003-2697(84)90006-x.
PMID:6206744
Abstract
Protein content of a wide variety of sample types may be determined by adsorption of microliter samples onto nitrocellulose, followed by amido black staining and laser densitometry. The method is quite rapid, sensitive, and selective, and dispenses with precipitation and transfer steps.
摘要
多种样品类型的蛋白质含量可通过将微升样品吸附到硝酸纤维素上，然后进行酰胺黑染色和激光密度测定来确定。该方法相当快速、灵敏且具有选择性，无需沉淀和转移步骤。
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