Ballesteros F, González-López J, de la Rubia T, Moreno J, Aneiros J, Ramos-Cormenzana A
Department of Microbiology, Faculty of Pharmacy, University of Granada, Spain.
Ann Inst Pasteur Microbiol (1985). 1986 Jan-Feb;137A(1):55-64. doi: 10.1016/s0769-2609(86)80005-9.
Azotobacter vinelandii ATCC 12837 cultured in dialysed soil medium with addition of 0.5% glucose showed four distinct morphological cell types: large cells, precyst forms, mature cysts and filterable corpuscles (0.3 micron in diameter). These results indicate that Azotobacter is a bacterium with a complex life cycle under certain culture conditions. Intracellular levels of RNA and poly-beta-hydroxybutyric acid were significantly affected when cells grown in dialysed soil were compared with those obtained after growth on defined medium (N-free). Further studies showed that the chemical composition of filterable corpuscles obtained from dialysed soil medium were different from the composition of normal Azotobacter cells produced in both culture media (dialysed soil and defined media). We suggest that filterable corpuscles represent a stage in the life cycle of Azotobacter in their natural environment.
在添加了0.5%葡萄糖的透析土壤培养基中培养的棕色固氮菌ATCC 12837表现出四种不同形态的细胞类型:大细胞、前孢囊形态、成熟孢囊和可过滤小体(直径0.3微米)。这些结果表明,在特定培养条件下,固氮菌是一种具有复杂生命周期的细菌。当将在透析土壤中生长的细胞与在限定培养基(无氮)上生长后获得的细胞进行比较时,RNA和聚-β-羟基丁酸的细胞内水平受到显著影响。进一步的研究表明,从透析土壤培养基中获得的可过滤小体的化学成分与在两种培养基(透析土壤和限定培养基)中产生的正常固氮菌细胞的成分不同。我们认为,可过滤小体代表了固氮菌在其自然环境中生命周期的一个阶段。