Lin L P, Pankratz S, Sadoff H L
J Bacteriol. 1978 Aug;135(2):641-6. doi: 10.1128/jb.135.2.641-646.1978.
Dormant cysts of Azotobacter vinelandii germinated at 30 degrees C in Burk nitrogen-free media containing 1% glucose. Samples taken at intervals and examined by electron microscopy revealed that as germination progressed, vesicle-like and fibrillar structures became visible in the intine region. Lamellae associated with the cell membrane appeared in the central body at 6 h post-initiation of germination. Both electron micrographic and chemical analysis showed that the poly-beta-hydroxybutyrate content of cysts decreased significantly after 4 h of germination. Dormant cysts were resistant to sonic oscillation, but this property was lost during their conversion to metabolically active vegetative cells.
棕色固氮菌的休眠孢囊在含有1%葡萄糖的伯克无氮培养基中于30℃下萌发。每隔一段时间取样并通过电子显微镜检查发现，随着萌发的进行，内膜区域可见囊泡状和纤维状结构。萌发开始后6小时，与细胞膜相关的片层出现在中央体中。电子显微镜和化学分析均表明，孢囊的聚-β-羟基丁酸含量在萌发4小时后显著下降。休眠孢囊对声波振荡具有抗性，但在其转化为代谢活跃的营养细胞过程中这种特性丧失。
