Tsai J C, Aladegbami S L, Vela G R
J Bacteriol. 1979 Aug;139(2):639-45. doi: 10.1128/jb.139.2.639-645.1979.
Batch cultures of Azotobacter vinelandii grown in phosphate-deficient media were compared with control cultures grown in phosphate-sufficient media. Phosphate limitation was assessed by total cell yield and by growth kinetics. Although cell protein, nucleic acids, and early growth rate were unaffected by phosphate deficiency, cell wall structure, oxygen uptake, and cell viability were significantly affected. Also, phosphate-limited cells contained much larger amounts of poly-beta-hydroxybutyric acid but lower adenylate nucleotide energy charge than did control cells. The ratio of adenosine 5'-triphosphate to adenosine 5'-diphosphate was much lower in phosphate-deficient cells. The data indicate a substrate saving choice of three metabolic pathways available to this organism under different growth conditions.
将在缺磷培养基中培养的棕色固氮菌分批培养物与在磷充足培养基中培养的对照培养物进行比较。通过总细胞产量和生长动力学评估磷限制情况。尽管细胞蛋白质、核酸和早期生长速率不受磷缺乏的影响，但细胞壁结构、氧气摄取和细胞活力受到显著影响。此外，磷限制的细胞比对照细胞含有更多的聚-β-羟基丁酸，但腺苷酸能量电荷较低。缺磷细胞中腺苷 5'-三磷酸与腺苷 5'-二磷酸的比率要低得多。数据表明，在不同生长条件下，该生物体可利用三种代谢途径进行底物节约选择。
