烟草蚀纹病毒基因组中 NIb 基因的重定位。
Relocation of the NIb gene in the tobacco etch potyvirus genome.
机构信息
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, Valencia, Spain.
出版信息
J Virol. 2014 Apr;88(8):4586-90. doi: 10.1128/JVI.03336-13. Epub 2014 Jan 22.
Abstract
Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic positions of the Tobacco etch virus (TEV) polyprotein. Only viruses with NIb at the amino terminus of the polyprotein or in between P1 and HC-Pro were viable in tobacco plants.
摘要
马铃薯 Y 病毒属的病毒通过一个长的开放阅读框表达其大部分蛋白，该框翻译为一个大的多蛋白，由三种病毒蛋白酶加工。为了了解马铃薯 Y 病毒属基因组结构的限制，我们将病毒 RNA 依赖性 RNA 聚合酶（NIb）顺式元件定位到烟草蚀纹病毒（TEV）多蛋白的所有可能的内含子间位置。只有在多蛋白的氨基末端或在 P1 和 HC-Pro 之间具有 NIb 的病毒在烟草植物中是有活力的。
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