Esmaeilpour Tahereh, Zarei Mohmmad Reza, Bahmanpour Soghra, Aliabadi Elham, Hosseini Ahmad, Jaberipour Mansooreh
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Med Sci. 2014 Jan;39(1):20-8.
Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoospermic samples.
The expression of LDH-C was examined by quantitative real-time polymerase chain reaction (q-RT PCR) and western blotting after it was treated with optimized concentrations of FF and PAF in twenty asthenozoospermic samples. Also, LDH-C expression was evaluated in five normozoospermic samples.
Samples with 75% FF and 100 nM of PAF had an increase in their percentages of progressive and slowly motile sperms and a decrease in their percentages of non-progressive and non-motile sperms. Moreover, LDH-C mRNA transcripts were not changed following PAF and FF treatment, and LDH-C protein was detected in highly progressive motile specimens treated with FF in the asthenozoospermic samples. Furthermore, LDH-C expression was more detectable in the normal sperms.
Our results indicated that PAF had more beneficial effects than FF on sperm motility in the asthenozoospermic samples (P=0.0001), although the LDH-C expressions of the sperms were not changed significantly in both groups. We found no association between LDH-C expression and sperm motility after FF and PAF actions. This finding, however, requires further investigation. The fact that LDH-C protein was detected in the normozoospermic, but not asthenozoospermic, samples could be cited as a reason for the infertility in these patients.
卵泡液（FF）和血小板活化因子（PAF）在人工授精中的应用可提高精子活力。乳酸脱氢酶C（LDH-C）是精子活力的关键酶。本研究探讨了FF和PAF对精子活力指数及LDH-C表达的影响。此外，还比较了弱精子症和正常精子症样本中LDH-C的表达。
用优化浓度的FF和PAF处理20份弱精子症样本后，通过定量实时聚合酶链反应（q-RT PCR）和蛋白质印迹法检测LDH-C的表达。同时，对5份正常精子症样本中的LDH-C表达进行评估。
含有75% FF和100 nM PAF的样本中，进行性运动和缓慢运动精子的百分比增加，非进行性和不运动精子的百分比降低。此外，PAF和FF处理后，LDH-C mRNA转录本未发生变化，在弱精子症样本中经FF处理的高进行性运动标本中检测到LDH-C蛋白。此外，正常精子中LDH-C的表达更易检测到。
我们的结果表明，在弱精子症样本中，PAF对精子活力的有益作用比FF更大（P = 0.0001），尽管两组精子的LDH-C表达均未显著改变。我们发现FF和PAF作用后，LDH-C表达与精子活力之间无关联。然而，这一发现需要进一步研究。在正常精子症而非弱精子症样本中检测到LDH-C蛋白这一事实，可被视为这些患者不孕的一个原因。
