Infertility Treatment Center of ACECR, Kermanshah, Iran.
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
JBRA Assist Reprod. 2023 Sep 12;27(3):482-489. doi: 10.5935/1518-0557.20230038.
Human sperm motility and hyperactivation (HA) are induced by different factors such as intracellular calcium concentration. Repaglinide is an antidiabetic drug that, via the blocking of ATP-sensitive potassium channels (K-ATP channels), depolarization of the β-cell membrane, and opening of the voltage-gated calcium channels leads to an increase in intracellular calcium. The present study aimed to examine the effects of repaglinide on in vitro sperm motility parameters, viability, and DNA integrity in normozoospermic and asthenozoospermic men.
Semen samples were collected from two groups of normozoospermic donors and asthenozoospermic patients. The samples were washed free of seminal plasma and then treated with medium alone (control) or with 100 nM and 1µM concentrations of repaglinide. After 1 h of incubation, percent sperm motility and hyperactivation were assessed; after 2 h of incubation, sperm viability and DNA fragmentation rate were evaluated by the Eosin-Y and acridine orange staining, respectively.
In both groups, repaglinide at a concentration of 100 nM and 1µM significantly improved percent sperm motility, hyperactivation, and vital sperms with normal DNA; in specimens from normozoospermic men, the 1µM concentration had a noticeable effect on progressive motility; in samples from asthenozoospermic men, the highest hyperactivation rate was seen at a concentration of 100 nM as compared with the 1µM concentration and controls (p<0.05).
Our results suggest that repaglinide can improve sperm motility, hyperactivity, viability, and DNA integrity in both normozoospermic and asthenozoospermic men.
人类精子的运动能力和超激活（HA）是由不同的因素诱导的，如细胞内钙离子浓度。瑞格列奈是一种抗糖尿病药物，通过阻断 ATP 敏感性钾通道（K-ATP 通道）、β细胞膜去极化和电压门控钙通道的开放，导致细胞内钙离子增加。本研究旨在研究瑞格列奈对正常精子和弱精子症患者体外精子运动参数、活力和 DNA 完整性的影响。
从两组正常精子供体和弱精子症患者中收集精液样本。将样本洗脱去精液，然后用单独的培养基（对照）或 100 nM 和 1µM 浓度的瑞格列奈处理。孵育 1 小时后，评估精子运动百分比和超激活率；孵育 2 小时后，通过伊红 Y 和吖啶橙染色分别评估精子活力和 DNA 碎片率。
在两组中，100 nM 和 1µM 浓度的瑞格列奈均显著提高了精子运动百分比、超激活率和具有正常 DNA 的活精子；在正常精子组的标本中，1µM 浓度对前向运动有明显影响；在弱精子症组的标本中，与 1µM 浓度和对照组相比，100 nM 浓度时出现了最高的超激活率（p<0.05）。
我们的结果表明，瑞格列奈可以改善正常精子和弱精子症患者的精子运动能力、超活性、活力和 DNA 完整性。
