Jarvi K, Roberts K D, Langlais J, Gagnon C
Urology Research Laboratory, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Fertil Steril. 1993 Jun;59(6):1266-75. doi: 10.1016/s0015-0282(16)55988-3.
To determine the effect of platelet-activating factor (PAF), the PAF derivative lyso-PAF, and lysophosphatidylcholine on in vitro sperm motility and to determine the role of albumin in this interaction.
Washed human spermatozoa were exposed to a range of PAF, lyso-PAF, or lysophosphatidylcholine concentrations, supplemented with different albumin concentrations, and the effect on sperm motion was quantified with a computer-assisted motion analysis. The metabolism of these compounds by spermatozoa was also assessed.
University research laboratory.
PATIENTS, PARTICIPANTS: Semen samples were obtained from donors and patients attending an infertility clinic.
Human spermatozoa were incubated with PAF, lyso-PAF, or lysophosphatidylcholine at 10(-11) to 6 x 10(-4) M, with 0% to 1.2% albumin, and motility was evaluated at different time periods from 5 to 240 minutes. Tritiated PAF, lyso-PAF, or lysophosphatidylcholine was incubated with spermatozoa, and the metabolites were separated and quantified by thin-layer chromatography (TLC).
Sperm motion characteristics, including the percentage of motile spermatozoa, velocity, and linearity, and sperm viability were determined. The metabolism of PAF, lyso-PAF, and lysophosphatidylcholine by spermatozoa was also studied.
Fifty micromolar of PAF and 100 microM lyso-PAF, supplemented with 0.3% albumin, increased sperm linear velocity by 41% +/- 5% (+/- SEM) and 44% +/- 5% and curvilinear velocity by 17% +/- 3% and 21 +/- 3%, respectively. Lysophosphatidylcholine had a similar effect but only at 22 degrees C and not 37 degrees C. In the absence of albumin, neither PAF, lyso-PAF, or lysophosphatidylcholine induced increases in sperm motion. Lysophosphatidylcholine and lyso-PAF are not detectably metabolized by spermatozoa, whereas 12.5% +/- 1.2% of PAF is hydrolyzed to lyso-PAF in 1 hour.
Platelet-activating factor, lyso-PAF, and lysophosphatidylcholine independently stimulate sperm linear and curvilinear velocity. This action requires albumin. These compounds may be of use in the treatment of asthenozoospermic males.
确定血小板活化因子（PAF）、PAF衍生物溶血PAF和溶血磷脂酰胆碱对体外精子活力的影响，并确定白蛋白在这种相互作用中的作用。
将洗涤后的人类精子暴露于一系列PAF、溶血PAF或溶血磷脂酰胆碱浓度下，补充不同浓度的白蛋白，并用计算机辅助运动分析对精子运动的影响进行量化。还评估了精子对这些化合物的代谢情况。
大学研究实验室。
患者、参与者：精液样本取自捐赠者和不孕不育诊所的患者。
将人类精子与浓度为10⁻¹¹至6×10⁻⁴M的PAF、溶血PAF或溶血磷脂酰胆碱一起孵育，白蛋白浓度为0%至1.2%，并在5至240分钟的不同时间段评估精子活力。将氚标记的PAF、溶血PAF或溶血磷脂酰胆碱与精子一起孵育，代谢产物通过薄层色谱法（TLC）分离并定量。
测定精子运动特征，包括活动精子百分比、速度和直线性，以及精子活力。还研究了精子对PAF、溶血PAF和溶血磷脂酰胆碱的代谢情况。
补充0.3%白蛋白时，50微摩尔的PAF和100微摩尔的溶血PAF分别使精子直线速度提高41%±5%（±标准误）和44%±5%，曲线速度提高17%±3%和21±3%。溶血磷脂酰胆碱有类似作用，但仅在22℃时有效，在37℃时无效。在无白蛋白的情况下，PAF、溶血PAF或溶血磷脂酰胆碱均未引起精子运动增加。精子无法检测到对溶血磷脂酰胆碱和溶血PAF的代谢，而PAF在1小时内有12.5%±1.2%水解为溶血PAF。
血小板活化因子、溶血PAF和溶血磷脂酰胆碱可独立刺激精子的直线和曲线速度。这一作用需要白蛋白。这些化合物可能用于治疗弱精子症男性。
