Kiefel V, Santoso S, Weisheit M, Müeller-Eckhardt C
Institute of Clinical Immunology and Blood Transfusion, Justus-Liebig-University, Giessen, FRG.
Blood. 1987 Dec;70(6):1722-6.
The analysis of sera containing different platelet-reactive antibodies, eg, autoantibodies, platelet-specific alloantibodies like anti-PIA1, -PIA2, -Baka, and HLA antibodies, is still difficult. Recently, monoclonal antibodies against major platelet membrane constituents (glycoproteins IIb/IIIa and Ib and HLA class I molecule) have become available. In this report we describe a new assay that takes advantage of these highly specific reagents to investigate selectively platelet reactive antibodies against epitopes on different glycoproteins. The reliability and specificity of this assay is demonstrated with known platelet-reactive autoantibodies and alloantibodies (anti-PIA1, -Baka, -Pen). The discovery of a PIA2 antibody in a serum of a polytransfused patient underscores the efficiency of this technique. Possible applications of this assay are discussed in detail.
对含有不同血小板反应性抗体（如自身抗体、血小板特异性同种抗体，如抗PIA1、-PIA2、-Baka和HLA抗体）的血清进行分析仍然很困难。最近，针对主要血小板膜成分（糖蛋白IIb/IIIa、Ib和HLA I类分子）的单克隆抗体已经问世。在本报告中，我们描述了一种新的检测方法，该方法利用这些高度特异性的试剂来选择性地研究针对不同糖蛋白上抗原决定簇的血小板反应性抗体。已知的血小板反应性自身抗体和同种抗体（抗PIA1、-Baka、-Pen）证明了该检测方法的可靠性和特异性。在一名多次输血患者的血清中发现PIA2抗体，突出了该技术的有效性。本文详细讨论了该检测方法的可能应用。
