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通过间接免疫荧光法自动定量抗核自身抗体的原始方法。

Original approach for automated quantification of antinuclear autoantibodies by indirect immunofluorescence.

作者信息

Bertin Daniel, Jourde-Chiche Noémie, Bongrand Pierre, Bardin Nathalie

机构信息

Aix-Marseille Université, Laboratoire d'Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 147 boulevard Baille, 13005 Marseille, France.

Aix-Marseille Université, Centre de Néphrologie et Transplantation Rénale, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.

出版信息

Clin Dev Immunol. 2013;2013:182172. doi: 10.1155/2013/182172. Epub 2013 Dec 18.

DOI:10.1155/2013/182172
PMID:24454469
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3878279/
Abstract

INTRODUCTION

Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases. For the discrimination of positive and negative samples, we propose here an original approach named Immunofluorescence for Computed Antinuclear antibody Rational Evaluation (ICARE) based on the calculation of a fluorescence index (FI).

METHODS

We made comparison between FI and visual evaluations on 237 consecutive samples and on a cohort of 25 patients with SLE.

RESULTS

We obtained very good technical performance of FI (95% sensitivity, 98% specificity, and a kappa of 0.92), even in a subgroup of weakly positive samples. A significant correlation between quantification of FI and IIF ANA titers was found (Spearman's ρ = 0.80, P < 0.0001). Clinical performance of ICARE was validated on a cohort of patients with SLE corroborating the fact that FI could represent an attractive alternative for the evaluation of antibody titer.

CONCLUSION

Our results represent a major step for automated quantification of IIF ANA, opening attractive perspectives such as rapid sample screening and laboratory standardization.

摘要

引言

间接免疫荧光法（IIF）是检测抗核抗体（ANA）的金标准方法，而抗核抗体是诊断系统性自身免疫性风湿病的重要标志物。为了区分阳性和阴性样本，我们在此提出一种名为“基于计算机的抗核抗体合理评估免疫荧光法（ICARE）”的原创方法，该方法基于荧光指数（FI）的计算。

方法

我们对237份连续样本以及一组25例系统性红斑狼疮（SLE）患者的样本进行了FI与视觉评估之间的比较。

结果

即使在弱阳性样本亚组中，我们也获得了FI非常好的技术性能（灵敏度95%，特异性98%，kappa值为0.92）。发现FI定量与IIF ANA滴度之间存在显著相关性（Spearman相关系数ρ = 0.80，P < 0.0001）。在一组SLE患者中验证了ICARE的临床性能，证实了FI可作为评估抗体滴度的一种有吸引力的替代方法。

结论

我们的结果代表了IIF ANA自动化定量的重要一步，开启了诸如快速样本筛查和实验室标准化等有吸引力的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/f8084df05887/CDI2013-182172.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/bc32c58fb0b2/CDI2013-182172.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/43514947c746/CDI2013-182172.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/0979c5e54692/CDI2013-182172.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/70cab2bc69ad/CDI2013-182172.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/f8084df05887/CDI2013-182172.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/bc32c58fb0b2/CDI2013-182172.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/43514947c746/CDI2013-182172.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/0979c5e54692/CDI2013-182172.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/70cab2bc69ad/CDI2013-182172.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce05/3878279/f8084df05887/CDI2013-182172.005.jpg

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