Immunohistochemical demonstration of coexistence of enkephalin-and substance P-like immunoreactivities in axonal components in the lumbar segments of cat spinal cord.

Coexistence of enkephalin-like and substance P-like immunoreactivities (ENK-LI and SP-LI) was shown in axonal components in the lumbar segments of cat spinal cord by using the double immunofluorescence method. Although axonal components labeled with both ENK-LI and SP-LI were distributed throughout the spinal gray, they were most dense in the ventral horn, and most sparse in the superficial part of the dorsal horn.