Mitchell B S, Stauber V V
Faculty of Medicine, University of Southampton, UK.
Histochem J. 1993 Feb;25(2):144-9. doi: 10.1007/BF00157987.
Immunoreactivities (IR) of substance P and leucine enkephalin have been demonstrated in the guinea-pig paracervical ganglion by an immunogold electron microscope method. Both substance P-IR and leucine enkephalin-IR were detected in large synaptic vesicles with electron-dense cores. The former neuropeptide was detected in nerve terminals and varicosities comprised mainly of large vesicles with electron-dense cores; the latter was detected in nerve terminals and varicosities that also included small, clear synaptic vesicles. In a minority of nerve terminals and varicosities coexistence of both immunoreactivities could be demonstrated within vesicles with an electron-dense core. Also present in these nerve terminals and varicosities were small, clear synaptic vesicles, though these were unreactive.
采用免疫金电子显微镜法已在豚鼠子宫颈旁神经节中证实了P物质和亮氨酸脑啡肽的免疫反应性（IR）。P物质免疫反应性和亮氨酸脑啡肽免疫反应性均在具有电子致密核心的大突触小泡中检测到。前一种神经肽在主要由具有电子致密核心的大泡组成的神经末梢和膨体中检测到；后一种在神经末梢和膨体中检测到，其中也包括小的、清亮的突触小泡。在少数神经末梢和膨体中，两种免疫反应性可在具有电子致密核心的小泡内共存。这些神经末梢和膨体中也存在小的、清亮的突触小泡，不过这些小泡无反应性。
