Department of Cardiology, Geriatric Cardiovascular Disease Research and Treatment Center, No. 252 Hospital of PLA, Baoding 071000, China.
Oxid Med Cell Longev. 2013;2013:507832. doi: 10.1155/2013/507832. Epub 2013 Dec 18.
To investigate the protection of salidroside of the heart against acute exhaustive injury and its mechanism of antioxidative stress and MAPKs signal transduction.
Adult male SD rats were divided into four groups randomly. Cardiomyocytes ultrastructure was observed by optical microscopy and transmission electron microscopy. The contents of CK, CK-MB, LDH, MDA, and SOD were determined by ELISA method, and the phosphorylation degrees of ERK and p38 MAPK were assayed by Western blotting. Cardiac function of isolated rat heart ischemia/reperfusion was detected by Langendorff technique.
Salidroside reduced the myocardium ultrastructure injury caused by exhaustive swimming, decreased the contents of CK, CK-MB, and LDH, improved the LVDP, ±LV dp/dt(max) under the basic condition, reduced the content of MDA and the phosphorylation degree of p38 MAPK, and increased the content of SOD and the phosphorylation degree of ERK in acute exhaustive rats.
Salidroside has the protection of the heart against acute exhaustive injury. The cardioprotection is mainly mediated by antioxidative stress and MAPKs signal transduction through reducing the content of MDA, increasing the content of SOD, and increasing p-ERK and decreasing p-p38 protein expressions in rat myocardium, which might be the mechanisms of the cardioprotective effect of salidroside.
探讨红景天苷对急性力竭性损伤心脏的保护作用及其抗氧化应激和 MAPKs 信号转导机制。
成年雄性 SD 大鼠随机分为 4 组。光学显微镜和透射电镜观察心肌超微结构。采用 ELISA 法测定 CK、CK-MB、LDH、MDA 和 SOD 的含量，Western blot 法测定 ERK 和 p38 MAPK 的磷酸化程度。采用 Langendorff 技术检测离体大鼠心脏缺血/再灌注的心脏功能。
红景天苷减轻了力竭游泳引起的心肌超微结构损伤，降低了 CK、CK-MB 和 LDH 的含量，提高了基础条件下的 LVDP、±LV dp/dt(max)，降低了 MDA 含量和 p38 MAPK 的磷酸化程度，增加了 SOD 的含量和 ERK 的磷酸化程度。
红景天苷对急性力竭性损伤心脏具有保护作用。这种心脏保护作用主要通过降低 MDA 含量、增加 SOD 含量以及增加大鼠心肌中 p-ERK 和减少 p-p38 蛋白表达来介导抗氧化应激和 MAPKs 信号转导，这可能是红景天苷心脏保护作用的机制。
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