Huang Shih-Chung, Lee Fang-Tsai, Kuo Tz-Yin, Yang Joan-Hwa, Chien Chiang-Ting
Departments of Cardiology, Kuang-Tien General Hospital, Taichung, Republic of China.
Chin J Physiol. 2009 Oct 31;52(5):316-24. doi: 10.4077/cjp.2009.amh029.
Rhodiola rosea improves exercise endurance and fatigue. We hypothesized that ingredients in Rhodiola rosea may increase antioxidant capability against swimming induced oxidative stress. In this study, we have identified the Rhodiola rosea ingredients, p-tyrosol, salidroside, rosin, rosavin and rosarin by high performance liquid chromatography-mass spectrometer and evaluated their O2(-), H2O2, and HOCl scavenging activities by a chemiluminescence analyzer. We next explored the effect and mechanism of Rhodiola rosea on 90-min swimming-induced oxidative stress in male Wistar rats fed with three doses of Rhodiola rosea extracts in drinking water (5, 25, 125 mg/day/rat) for 4 weeks. Our results showed that the 4 major ingredients (salidroside, rosin, rosavin and rosarin) from Rhodiola rosea extracts scavenged O2(-), H2O2, and HOCl activity in a dose-dependent manner. The ninety-min swimming exercise increased the O2(-)* production in the order: liver > skeletal muscle > blood, indicating that liver is the most sensitive target organ. The level of plasma malonedialdehyde, a lipid peroxidation product, was also increased after exercise. Treatment of 4 weeks of Rhodiola rosea extracts significantly inhibited swimming exercise-enhanced O2(-)* production in the blood, liver and skeletal muscle and plasma malonedialdehyde concentration. The expression in Mn-superoxide dismutase Cu/Zn-superoxide dismutase, and catalase in livers were all enhanced after 4 weeks of Rhodiola rosea supplementation especially at the dose of 125 mg/day/rat. Treatment of Rhodiola rosea extracts for 4 weeks significantly increased swimming performance. In conclusion, treatment of Rhodiola rosea extracts for 4 weeks could reduce swimming-enhanced oxidative stress possibly via the reactive oxygen species scavenging capability and the enhancement of the antioxidant defense mechanisms.
红景天可提高运动耐力并减轻疲劳。我们推测红景天中的成分可能会增强对游泳诱导的氧化应激的抗氧化能力。在本研究中，我们通过高效液相色谱-质谱仪鉴定了红景天中的成分对香豆酸、红景天苷、迷迭香酸、rosavin和rosarin，并使用化学发光分析仪评估了它们对超氧阴离子（O2(-)）、过氧化氢（H2O2）和次氯酸（HOCl）的清除活性。接下来，我们研究了给雄性Wistar大鼠在饮用水中喂食三种剂量的红景天提取物（5、25、125毫克/天/只），持续4周后，红景天对90分钟游泳诱导的氧化应激的影响及其机制。我们的结果表明，红景天提取物中的4种主要成分（红景天苷、迷迭香酸、rosavin和rosarin）以剂量依赖的方式清除O2(-)、H2O2和HOCl活性。90分钟的游泳运动使O2(-)*的产生量按以下顺序增加：肝脏>骨骼肌>血液，表明肝脏是最敏感的靶器官。运动后血浆中脂质过氧化产物丙二醛的水平也升高。红景天提取物处理4周可显著抑制游泳运动增强的血液、肝脏和骨骼肌中O2(-)*的产生以及血浆丙二醛浓度。补充红景天4周后，肝脏中锰超氧化物歧化酶、铜/锌超氧化物歧化酶和过氧化氢酶的表达均增强，尤其是在剂量为125毫克/天/只时。红景天提取物处理4周可显著提高游泳能力。总之，红景天提取物处理4周可通过清除活性氧的能力和增强抗氧化防御机制来减轻游泳增强的氧化应激。
