Ning S C, Zhang X Z, Qiu W L, He R G
Ninth People's Hospital, Shanghai Second Medical University.
Zhonghua Zhong Liu Za Zhi. 1987 Jul;9(4):273-5.
This paper reports an observation on kinetics of induction and resolution of the thermotolerance expressed by Tca8113 in vitro in form of colony-forming rate, synchronization and laser flow cytometry after the cells being exposed to continuous and fractionized heat treatment of 41.5-45.5 degrees C for 4 hours. The process of thermotolerance kinetics could be divided into three phases: triggering, development and decay. The triggering phase lasted about 8 hours. The duration of development and decay phases was related to the temperature of initial heating. The higher the temperature, the shorter the duration. Generally, thermotolerance gradually disappeared 96 hours after the initial heating treatment. The thermotolerance of the synchronized cells in various phases was G1 greater than G2 greater than M greater than S. Hyperthermia simultaneously combined with pingyangmycin both produces a greater synergetic cytotoxic effect and reduces the degree of thermotolerance.
本文报道了Tca8113细胞在体外经41.5 - 45.5℃连续和分次热处理4小时后，以集落形成率、同步化及激光流式细胞术的形式对其表达的热耐受性诱导和消退动力学的观察。热耐受性动力学过程可分为三个阶段：触发、发展和消退。触发阶段持续约8小时。发展和消退阶段的持续时间与初始加热温度有关。温度越高，持续时间越短。一般来说，热耐受性在初始热处理后96小时逐渐消失。处于不同阶段的同步化细胞的热耐受性为G1期＞G2期＞M期＞S期。热疗同时联合平阳霉素既产生更大的协同细胞毒性作用，又降低热耐受性程度。
