Fox M H, Read R A, Bedford J S
Radiat Res. 1985 Dec;104(3):429-42.
We have extended our studies on the cell cycle dependence of thermotolerance to include HeLa cells heated at 45.0 degrees C to compare the results to Chinese hamster ovary (CHO) cells. We found that asynchronous HeLa cells were more resistant to heat than CHO cells but showed a similar development and decay of thermotolerance. Flow cytometry (FCM) was used to study redistributions in the cell cycle after an initial heat dose. Cells heated for 35 min at 45.0 degrees C were delayed in G1 by about 7 h compared to controls, with delays in late S and G2/M phase also. The heat sensitivity varied through the cell cycle; G1 cells were the most resistant to heat, while S-phase cells were uniformly sensitive throughout S phase, and G2 cells were resistant. Thermotolerance could be induced and expressed in early or late S-phase cells, but to a lesser extent than for G1 cells. The results were similar in many respects to CHO cells, but there were significant differences.
我们将对热耐受性的细胞周期依赖性研究扩展到了对在45.0摄氏度加热的HeLa细胞的研究，以便将结果与中国仓鼠卵巢（CHO）细胞进行比较。我们发现，异步HeLa细胞比CHO细胞对热更具抗性，但表现出相似的热耐受性发展和衰退过程。使用流式细胞术（FCM）研究初始热剂量后细胞周期的重新分布。与对照组相比，在45.0摄氏度加热35分钟的细胞在G1期延迟约7小时，在S期后期和G2/M期也有延迟。热敏感性在整个细胞周期中有所不同；G1期细胞对热最具抗性，而S期细胞在整个S期均一敏感，G2期细胞则具有抗性。热耐受性可在S期早期或晚期细胞中诱导并表达，但程度低于G1期细胞。结果在许多方面与CHO细胞相似，但也存在显著差异。
