Kaczmarek L, Calabretta B, Elfenbein I B, Mercer W E
Department of Pathology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Exp Cell Res. 1987 Nov;173(1):70-9. doi: 10.1016/0014-4827(87)90332-6.
We have studied the cell cycle of resting T lymphocytes from long-term (LT) cultures following stimulation with phytohemagglutinin (PHA) and recombinant Interleukin 2 (IL-2). We examined the kinetics of entry into S phase by autoradiography, the accumulation of cellular RNA by microfluorometric techniques, and ultrastructural morphology by electron microscopy. In addition, we examined the expression at the mRNA level of six cell cycle-dependent growth-regulated genes (c-fos, c-myc, KC-1, JE-3, vimentin, and histone H3). We show that T lymphocytes of LT cultures respond differently to mitogenic stimulation than the T lymphocytes of freshly isolated peripheral blood mononuclear cell cultures. At the ultrastructural, biochemical, and molecular levels, resting T lymphocytes of LT cultures can be distinguished from physiological (G0) lymphocytes of peripheral blood.
我们研究了长期（LT）培养的静息T淋巴细胞在用植物血凝素（PHA）和重组白细胞介素2（IL-2）刺激后的细胞周期。我们通过放射自显影检查进入S期的动力学，通过微量荧光测定技术检查细胞RNA的积累，并通过电子显微镜检查超微结构形态。此外，我们检查了六个细胞周期依赖性生长调节基因（c-fos、c-myc、KC-1、JE-3、波形蛋白和组蛋白H3）在mRNA水平的表达。我们发现，LT培养的T淋巴细胞对有丝分裂刺激的反应与新鲜分离的外周血单个核细胞培养的T淋巴细胞不同。在超微结构、生化和分子水平上，LT培养的静息T淋巴细胞可与外周血的生理性（G0）淋巴细胞区分开来。
