Kaczmarek L, Calabretta B, Baserga R
Biochem Biophys Res Commun. 1985 Dec 17;133(2):410-6. doi: 10.1016/0006-291x(85)90921-0.
We have studied the expression of seven cell cycle-dependent genes in phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells, in macrophage-depleted cultures and in macrophage-depleted cultures plus Interleukin-2 (IL-2). The expression of all seven genes is increased in PHA stimulated peripheral cells. Only two (2F1 and the IL-2 receptor) are increased in PHA-stimulated macrophage depleted cultures. Addition of IL-2 to these cultures increased the RNA levels of four genes (KC-1, c-myc, beta-actin and IL-2R), but has no effect on three others (4F1, 2F1, and JE-3). The results indicate that the expression of these cell cycle genes is regulated by different components of the mitogenic stimulus.
我们研究了七种细胞周期依赖性基因在植物血凝素（PHA）刺激的外周血单核细胞、巨噬细胞耗竭培养物以及巨噬细胞耗竭培养物加白细胞介素-2（IL-2）中的表达情况。在PHA刺激的外周细胞中，所有七种基因的表达均增加。在PHA刺激的巨噬细胞耗竭培养物中，只有两种基因（2F1和IL-2受体）表达增加。向这些培养物中添加IL-2可使四种基因（KC-1、c-myc、β-肌动蛋白和IL-2R）的RNA水平升高，但对其他三种基因（4F1、2F1和JE-3）没有影响。结果表明，这些细胞周期基因的表达受促有丝分裂刺激的不同成分调控。
