Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China; Joint International Research Lab of Lignocellulosic Functional Materials, Nanjing Forestry University, Nanjing 210037, China.
Int J Biol Macromol. 2022 Nov 30;221:38-47. doi: 10.1016/j.ijbiomac.2022.08.193. Epub 2022 Sep 5.
In this work, five different NaOH-catalyzed ethylene glycol (EG) pretreatments together with water-soluble sulfonated lignin (SL) were used for enhancing sugarcane bagasse (SCB) enzymatic digestion. The results showed that the coupling of NaOH and EG into a one-pot pretreatment (10%NaOH/EG) was more beneficial to improve SCB enzymatic hydrolysis than that of single 10%NaOH or EG pretreatment, or the two-step pretreatment of NaOH and EG in different sequence (10%NaOH+EG and EG + 10%NaOH, respectively). The highest glucose yield of this work was 91.2 %, mainly released from the SCB that pretreated with 10%NaOH/EG at 130 °C for 60 min and 72 h enzymatic hydrolysis. The adding of SL into the enzymatic hydrolysis step could significantly lower the cellulase dosage and hydrolysis time from 20 FPU/g and 72 h to 10 FPU/g and 24 h, respectively, meanwhile keeping a high glucose yield of 90.4 %. The characterization of various pretreated or un-pretreated SCB confirmed that the improvement of hydrolysis efficiency of SCB after 10%NaOH/EG pretreatment was closely related to the removal of various components barriers in SCB and the fragmentation of pretreated solid. It can be concluded that the developed NaOH-catalyzed ethylene glycol pretreatment was an efficiency way to enhance the sugar release from SCB.
在这项工作中，使用了五种不同的氢氧化钠催化乙二醇（EG）预处理方法，同时加入水溶性磺化木质素（SL），以提高甘蔗渣（SCB）的酶解效率。结果表明，NaOH 和 EG 一锅法预处理（10%NaOH/EG）比单一 10%NaOH 或 EG 预处理，或 NaOH 和 EG 以不同顺序两步预处理（10%NaOH+EG 和 EG+10%NaOH）更有利于提高 SCB 的酶解效率。该工作获得的最高葡萄糖得率为 91.2%，主要来自于在 130°C 下用 10%NaOH/EG 预处理 60 分钟，然后进行 72 小时酶解的 SCB。在酶解步骤中添加 SL 可以显著降低纤维素酶的用量和水解时间，从 20 FPU/g 和 72 小时分别降低到 10 FPU/g 和 24 小时，同时保持 90.4%的高葡萄糖得率。对各种预处理或未预处理的 SCB 的特性分析表明，10%NaOH/EG 预处理后 SCB 水解效率的提高与 SCB 中各种成分障碍的去除和预处理固体的碎片化密切相关。可以得出结论，开发的氢氧化钠催化乙二醇预处理是一种提高 SCB 中糖释放效率的有效方法。
