Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil.
Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, 12602-810, Lorena, SP, Brazil.
Sci Rep. 2017 Jun 20;7(1):3893. doi: 10.1038/s41598-017-04262-y.
Low cost and high efficiency cellulolytic cocktails can consolidate lignocellulosic ethanol technologies. Sugarcane bagasse (SCB) is a low cost agro-industrial residue, and its use as a carbon source can reduce the costs of fungi cultivation for enzyme production. Chrysoporthe cubensis grown under solid state fermentation (SSF) with wheat bran has potential to produce efficient enzymatic extracts for SCB saccharification. This fungus was grown under submersed fermentation (SmF) and SSF with in natura SCB, pretreated with acid or alkali and with others carbon sources. In natura SCB induced the highest carboxymethylcellulase (CMCase), xylanase, β-xylosidase, α-galactosidase and mannanase activities by C. cubensis under SSF. In natura and washed SCB, inducers of enzyme production under SSF, did not induce high cellulases and hemicellulases production by C. cubensis in SmF. The C. cubensis enzymatic extract produced under SSF with in natura SCB as a carbon source was more efficient for lignocelulolic biomass hydrolysis than extracts produced under SSF with wheat bran and commercial cellulolytic extract. Chrysoporthe cubensis showed high potential for cellulases and hemicellulases production, especially when grown under SSF with in natura SCB as carbon source.
低成本、高效率的纤维素酶混合物可以整合木质纤维素乙醇技术。甘蔗渣（SCB）是一种低成本的农业工业废料，将其用作碳源可以降低真菌培养酶生产的成本。在固态发酵（SSF）中用麦麸培养的 Chrysoporthe cubensis 具有生产高效酶提取物用于 SCB 糖化的潜力。该真菌在浸没发酵（SmF）和 SSF 中用天然 SCB、酸或碱预处理以及其他碳源进行培养。在 SSF 下，天然 SCB 诱导 Chrysoporthe cubensis 产生最高的羧甲基纤维素酶（CMCase）、木聚糖酶、β-木糖苷酶、α-半乳糖苷酶和甘露聚糖酶活性。天然和洗涤后的 SCB 作为 SSF 中的诱导物，不能诱导 C. cubensis 产生高纤维素酶和半纤维素酶。以天然 SCB 为碳源在 SSF 下生产的 C. cubensis 酶提取物比在 SSF 下用麦麸和商业纤维素酶提取物生产的酶提取物更有效地水解木质纤维素生物质。Chrysoporthe cubensis 显示出高产纤维素酶和半纤维素酶的潜力，尤其是在 SSF 中用天然 SCB 作为碳源培养时。
