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叶片中的亚硝酸盐还原;离体叶绿体研究。

Nitrite reduction in leaves; Studies on isolated chloroplasts.

机构信息

Department of Plant Science, Faculty of Agriculture, The University, NE1 7RU, Neweastle upon Tyne, UK.

出版信息

Planta. 1974 Sep;116(3):187-96. doi: 10.1007/BF00390226.

Abstract

Chloroplast preparations from spinach leaves containing a high percentage of intact chloroplasts were capable of light dependent nitrite reduction at rates around 9 μmol/mg chlorophyll/h for at least 50 min. This reduction was inhibited by DCMU but unaffected by uncouplers of photosynthetic phosphorylation. Nitrite reduction was not accompanied by a stoichiometric evolution of oxygen evolution. The disappearance of nitrite was accompanied by an approximately stoichiometric formation of reduced nitrogen.

摘要

菠菜叶片的叶绿体制剂中含有高比例的完整叶绿体,在光照下,这些叶绿体能够以 9 μmol/mg 叶绿素/h 左右的速率进行亚硝酸还原,至少持续 50 分钟。该还原过程被 DCMU 抑制,但不受光合磷酸化解偶联剂的影响。亚硝酸还原过程中没有伴随着氧释放的化学计量进化。亚硝酸的消失伴随着还原态氮的近似化学计量形成。

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